Western blot analysis of HMGCR expressed in Mouse spleen,Rat brain using HMGCR Rabbit pAb at 1:1000. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:5000. Lysates/proteins: 30ug per lane. Blocking buffer: 5% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 120s.
Western blot analysis of HMGCR expressed in U-251MG,HepG2,Rat brain using HMGCR Rabbit pAb at 1:1000. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:5000. Lysates/proteins: 30ug per lane. Blocking buffer: 5% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 120s.
Western blot analysis of HMGCR expressed in Mouse spleen,Rat brain using HMGCR Rabbit mAb at 1:1000. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:5000. Lysates/proteins: 30ug per lane. Blocking buffer: 5% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 120s.
AYD11989-wb1
Western blot detection of AMPK α1 in Hela ，nih-3T3 cell lysates using AMPK α1 antibody(1:1000 diluted).
Western blot analysis of Phospho-AMPKa1/AMPKa2-T183/T172 expressed in 293T,C2C12,C6 using Phospho-AMPKa1/AMPKa2-T183/T172 Rabbit pAb at 1:500. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:5000. Lysates/proteins: 30ug per lane. Blocking buffer: 5% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 120s.
Western blot analysis of Phospho-AMPKα1-S485 expressed in 293T using Phospho-AMPKα1-S485 Rabbit at 1:1000. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:5000. Lysates/proteins: 30ug per lane. Blocking buffer: 5% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 120s.
Western blot analysis of AMPK alpha 1 expressed in Mouse liver,Mouse brain,Rat liver using AMPK alpha 1 Rabbit mAb at 1:1000. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:5000. Lysates/proteins: 30ug per lane. Blocking buffer: 5% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 120s.
Western blot analysis of Phospho-AMPK alpha 1 (S496) expressed in Mouse liver,Mouse brain,Rat liver using Phospho-AMPK alpha 1 (S496) Rabbit mAb at 1:1000. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:5000. Lysates/proteins: 30ug per lane. Blocking buffer: 5% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 120s.
