| 体外研究 |
Coenzyme Q0 (0-40 µM; 24 h) and inhibits viability and growth of human ovarian carcinoma cells.
Coenzyme Q0 (CoQ0) (0-30 µM; 24 h; SKOV-3 cells) has anti-proliferative activity through induction of G2/M cell-cycle arrest and reduction of cell-cycle regulatory proteins.
Coenzyme Q0 (CoQ0) (0-30 µM; 0-30 min; SKOV-3 cells) increases intracellular ROS levels to promote SKOV-3 cell death.
Coenzyme Q0 (CoQ0) (0-30 µM; 24 h; SKOV-3 cells) induces autophagy by increase accumulation of LC3-II, GFP-LC3 puncta, AVOs formation and Beclin-1/Bcl-2 dysregulation.
Coenzyme Q0 (CoQ0) (0-30 µM; 24 h; SKOV-3 cells) induces apoptosis by mitochondrial (caspase-3, PARP and Bax/Bcl-2 dysregulation) and ER stress (caspase-12 and Hsp70) signals.
Coenzyme Q0 (CoQ0) (30 µM; 24 h; SKOV-3 cells) suppresses of HER-2/AKT/mTOR signaling to potentiate the apoptosis and autophagy mechanisms.
Coenzyme Q0 (CoQ0) (0-10 µM; 0.5-18 h; RAW264.7 cells) regulates NFκB/AP-1 activation and enhances Nrf2 stabilization.
Coenzyme Q0 (CoQ0) (5 µM; 0-12 h; EA.hy 926 cells) has anti-angiogenic activity in EA.hy 926 cells. The accuracy of these methods have not been independently confirmed. They are for reference only. Cell Viability Assay | Cell Line: | SKOV-3, A2780 and A2870/CP70 cells | | Concentration: | 0, 10, 20, 30 and 40 µM | | Incubation Time: | 24 hours | | Result: | Decreased viability with the IC50 values of 26.6 µM, 27.3 µM and 28.4 µM for SKOV-3, A2780 and A2870/CP70 cells, respectively. |
Cell Cycle Analysis | Cell Line: | SKOV-3, A2780 and A2870/CP70 cells | | Concentration: | 0, 10, 20 and 30 µM | | Incubation Time: | 24 hours | | Result: | Arrested cell cycle at G2/M phase and reduced cell-cycle proteins in SKOV-3 cells. |
Apoptosis Analysis | Cell Line: | SKOV-3, A2780 and A2870/CP70 cells | | Concentration: | 0, 5, 15 and 30 µM | | Incubation Time: | 24 hours | | Result: | Promoted the conversion of LC3–1 to LC3-II and increased the LC3-II accumulation. Increased Bax/Bcl-2 ratio in a dose-dependent manner. |
Apoptosis Analysis | Cell Line: | SKOV-3 cells | | Concentration: | 0, 10, 20 and 30 µM | | Incubation Time: | 24 hours | | Result: | Had the percentage of early apoptotic cells are 25.1%, 34% and 36% for 10, 20 and 30 µM, respectively. |
Western Blot Analysis | Cell Line: | SKOV-3 cells | | Concentration: | 0, 5, 15 and 30 µM | | Incubation Time: | 24 hours | | Result: | Activated of caspase-3 and cleavaged of PARP. Increased the expressions of caspase-12, HSP-70 and Bax in a dose-dependent manner, decreased the expressions of Bcl-2. |
Western Blot Analysis | Cell Line: | SKOV-3 cells | | Concentration: | 30 µM | | Incubation Time: | 24 hours | | Result: | Decreased the phosphorylated HER-2 (Y1221) levels, p-AKT (Ser473) and p-mTOR (S2448) levels. |
Western Blot Analysis | Cell Line: | RAW264.7 cells | | Concentration: | 0, 2.5, 5 and 10 µM | | Incubation Time: | 0.5-18 hours | | Result: | Inhibited iNOS/COX-2 protein expressions with reductions of NO, PGE2, TNF-α and IL-1β secretions. |
Western Blot Analysis | Cell Line: | EA.hy 926 cells | | Concentration: | 5 µM | | Incubation Time: | 0, 1, 3, 6 and 12 hours | | Result: | Increased expressions of heme oxygenase-1 (HO-1) and γ-glutamylcysteine synthetase (γ-GCLC), inhibits protein expressions of matrix metalloproteinase-9 (MMP-9), reduces TNF-α-induced nuclear translocation and transcriptional activation of nuclear factor-κB (NF-κB). |
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