ABI2 (YD13466) Rabbit mAb (货号:AYD16171)
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货号
AYD16171
靶点/基因
ABI2
宿主
Rabbit
克隆性
Monoclonal
反应种属
Human, Mouse, Rat
应用
IHC-P, ICC/IF
产品信息
应用指南
相关产品
抗原信息
靶点信息
资料与支持
常见问题
| 反应 | Human, Mouse, Rat |
|---|---|
| 宿主 | Rabbit |
| 克隆性 | Monoclonal |
| 同种型 | IgG |
| 应用 | IHC-PICC/IF |
| 推荐浓度 | |
| 理论分子量 | 56kDa |
| 实测分子量 | |
| 形式 | Liquid |
| 保存条件 | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.75% BSA,50% glycerol,pH7.3. |
| 偶联物 | Unconjugated |
| 阳性对照 | U-251MG,Mouse brain |
| 细胞定位 | Cytoplasm, Nucleus, Cell projection, lamellipodium, filopodium, cytoskeleton, Cell junction, adherens junction |
| 纯化 | 亲和纯化 |
应用与推荐条件
快速判断怎么用
以下条件基于推荐浓度、验证图说明与通用实验要求整理，可作为预实验起点；不同样本和检测体系建议做梯度优化。
IHC-P
IHC-P 推荐条件
| 推荐稀释 | 请参考验证图说明或咨询技术支持 |
|---|---|
| 建议样本/阳性对照 | U-251MG,Mouse brain |
| 关键条件 | 石蜡切片建议优化抗原修复液 pH、修复时间和一抗孵育条件 |
| 预期结果 | 预期定位：Cytoplasm, Nucleus, Cell projection, lamellipodium, filopodium, cytoskeleton, Cell junction, adherens junction |
| 对照设置 | 建议设置阳性样本、阴性样本和二抗/同型对照 |
ICC/IF
ICC/IF 推荐条件
| 推荐稀释 | 请参考验证图说明或咨询技术支持 |
|---|---|
| 建议样本/阳性对照 | U-251MG,Mouse brain |
| 关键条件 | 建议优化固定、通透和封闭条件，并设置二抗空白对照 |
| 预期结果 | 预期荧光定位：Cytoplasm, Nucleus, Cell projection, lamellipodium, filopodium, cytoskeleton, Cell junction, adherens junction |
| 对照设置 | 建议设置阳性样本、阴性样本和二抗/同型对照 |
相关产品
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抗原信息
| 抗原信息 | 请咨询 技术支持 |
|---|---|
| 序列 | Email For Sequence |
靶点信息
| 研究背景 | Regulator of actin cytoskeleton dynamics underlying cell motility and adhesion. Functions as a component of the WAVE complex, which activates actin nucleating machinery Arp2/3 to drive lamellipodia formation. Acts as regulator and substrate of nonreceptor tyrosine kinases ABL1 and ABL2 involved in processes linked to cell growth and differentiation. Positively regulates ABL1-mediated phosphorylation of ENAH, which is required for proper polymerization of nucleated actin filaments at the leading edge. Contributes to the regulation of actin assembly at the tips of neuron projections. In particular, controls dendritic spine morphogenesis and may promote dendritic spine specification toward large mushroom-type spines known as repositories of memory in the brain (By similarity. In hippocampal neurons, may mediate actin-dependent BDNF-NTRK2 early endocytic trafficking that triggers dendrite outgrowth (By similarity. Participates in ocular lens morphogenesis, likely by regulating lamellipodia-driven adherens junction formation at the epithelial cell-secondary lens fiber interface (By similarity. Also required for nascent adherens junction assembly in epithelial cells. |
|---|---|
| 基因 ID | 10152 |
| 基因名 | ABI2 |
| Swiss | Q9NYB9 |
| 别名 | ABI2 (YD13466),ABI2 (YD13466) Rabbit mAb,ABI2,Abelson interactor 2,Abl-binding protein 3,Arg-binding protein 1,ARGBPIA |
资料与技术支持
验证数据
1 张验证图
页面顶部轮播可查看应用验证图，覆盖 IHC-P / ICC/IF。
常见问题
当前页面标注应用包括 IHC-P, ICC/IF，建议结合页面验证图和推荐稀释比例进行预实验优化。
可通过页面询价/留言入口提交货号和批号，技术支持会协助提供对应批次资料。
页面推荐条件可作为起始浓度，不同样本、固定方式和检测体系可能需要梯度优化。