4-Nitrophenyl α-D-galactopyranoside  (货号:B26921)

说明书
4-Nitrophenyl α-D-galactopyranoside
规格价格
Free Sample (0.1 - 0.2 mg) 询价
500 mg 询价
1 g 询价
100 mg ￥600.00 加购物车
250 mg ￥1200.00 加购物车

货号：B26921

生物活性 4-Nitrophenyl α-D-galactopyranoside (PNP-alpha-D-Gal) is an artificial substrate of 4-nitrophenyl (pNP) glycopyranoside for detecting α-galactosidase activity. The amount of released pNP is significantly increased when 4-Nitrophenyl α-D-galactopyranoside is used as substrates.
CAS 7493-95-0
中文名称 4-硝基苯基 α-D-吡喃半乳糖苷
分子量 301.25
体外研究

Using 4-nitorophenol (pNP)-glycopyranosides as pseudosubstrates, the amount of liberated pNP from the 4-Nitrophenyl α-D-galactopyranoside is significantly higher than that from the other pNP-glycopyranosides at pH 4.0 whereas no activity was detected at pH 7.0.
The digestion assays using 4-nitrophenyl (pNP) glycopyranosides as artificial substrates shows that yieldin liberated galactose residues from 4-Nitrophenyl α-D-galactopyranoside mainly at pH 4.0, i.e. the pH level where yield threshold is decreased at most.

The accuracy of these methods have not been independently confirmed. They are for reference only.
体内研究
形式 Solid
运输条件 Room temperature in continental US; may vary elsewhere.
保存条件
溶解性
In Vitro: 

H2O : 16.67 mg/mL (55.34 mM; ultrasonic and warming and heat to 60°C)

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM3.3195 mL16.5975 mL33.1950 mL
5 mM0.6639 mL3.3195 mL6.6390 mL
10 mM0.3320 mL1.6598 mL3.3195 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂： PBS

    Solubility: 25 mg/mL (82.99 mM); Clear solution; Need ultrasonic

*以上所有助溶剂都可在 MCE 网站选购。

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