Western blot analysis of SUMO2/3 expressed in SH-SY5Y,293T,NIH/3T3 using SUMO2/3 Rabbit pAb at 1:1000. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:5000. Lysates/proteins: 30ug per lane. Blocking buffer: 5% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 120s.

Western blot analysis of SUMO2/3 expressed in Mouse kidney,PC-12,293T using SUMO2/3 Rabbit pAb at 1:1000. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:5000. Lysates/proteins: 30ug per lane. Blocking buffer: 5% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 120s.

Western blot analysis of Sumo2/3 expressed in human liver cancer,mouse brain,rat lung using Sumo2/3 Rabbit mAb at 1:1000. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:5000. Lysates/proteins: 30ug per lane. Blocking buffer: 5% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 120s.

AYD23774-wb1

AYD15908-wb1