Western blot analysis of RENT1/hUPF1 expressed in HeLa,C2C12,C6 using RENT1/hUPF1 Rabbit mAb at 1:1000. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:5000. Lysates/proteins: 30ug per lane. Blocking buffer: 5% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 120s.