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p-Nitrophenyl α-L-arabinopyranoside  (Cat.#:AYB27017)

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Cat.#:AYB27017

CAS:1223-07-0

Chinese name:4-硝基苯基Α-L-吡喃阿拉伯糖苷

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  • Biological activity

  • Information

Biological activity

Bioactivity p-Nitrophenyl α-L-arabinopyranoside is a biochemical reagent. p-Nitrophenyl α-L-arabinopyranoside can be hydrolyzed by recombinant BgaA (rBgaA, isolated from E. coli BL21 (DE3) strain harboring pEBGA29). p-Nitrophenyl α-L-arabinopyranoside has potential application in enzyme activity detection.
CAS 1223-07-0
Chinese name 4-硝基苯基Α-L-吡喃阿拉伯糖苷
Mol.Wt. 271.22
In vitro

p-Nitrophenyl α-L-arabinopyranoside 与 rBgaA 的亲和度 Km 值为 6.06 mM。
p-Nitrophenyl α-L-arabinopyranoside 对 xylosidase–arabinosidase (xarB) 基因具有高活性。
p-Nitrophenyl α-L-arabinopyranoside 在酶活性检测中的应用
(1) 200 µL 2 mM p-Nitrophenyl α-L-arabinopyranoside、100 µL 酶、300 µL 50 mM 磷酸盐缓冲液 (pH 7.0),在 37℃ 下共孵育 0.5、1 和 5 h。
(2) 加入 400 µL 0.5 M 氢氧化钠,终止反应。
(3) 在 405 nm 处测量混合物的吸光度。
注:在人参皂苷存在的情况下,用丁醇萃取终止反应。

The accuracy of these methods have not been independently confirmed. They are for reference only.

Transportation Room temperature in continental US; may vary elsewhere.
Storage Please store the product under the recommended conditions in the Certificate of Analysis.

Information

Intro p-Nitrophenyl α-L-arabinopyranoside 是一种生化试剂。p-Nitrophenyl α-L-arabinopyranoside 能被重组 BgaA (rBgaA,从含有 pEBGA29 的大肠杆菌 BL21 (DE3) 菌株中分离) 水解。p-Nitrophenyl α-L-arabinopyranoside 在酶活性检测中有潜在应用。
Synonym 4-硝基苯基Α-L-吡喃阿拉伯糖苷
AYB27017