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o-Phenanthroline monohydrate  (Cat.#:AYB26723)

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Cat.#:AYB26723

CAS:5144-89-8

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10 mM * 1 mL in DMSO ¥610.00 AddToCart
  • Biological activity

  • Information

Biological activity

Bioactivity o-Phenanthroline (1,10-Phenanthroline) monohydrate, a metal chelator, prevents the induction of chromosomal aberrations in streptozotocin-treated cells. o-Phenanthroline monohydrate forms a red chelate with Fe2+ that absorbs maximally at 510 nm. o-Phenanthroline (1,10-Phenanthroline) monohydrate is also a MMP inhibitor.
CAS 5144-89-8
Mol.Wt. 198.22
In vitro

o-Phenanthroline (1,10-Phenanthroline, 1mM) inhibits autolysis of sea cucumber body wall.

The accuracy of these methods have not been independently confirmed. They are for reference only.

Format Solid
Transportation Room temperature in continental US; may vary elsewhere.
Storage 4°C, sealed storage, away from moisture
Solubility
In Vitro: 

DMSO : 100 mg/mL (504.49 mM; Need ultrasonic)

H2O : 2 mg/mL (10.09 mM; ultrasonic and warming and heat to 60°C)

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM5.0449 mL25.2245 mL50.4490 mL
5 mM1.0090 mL5.0449 mL10.0898 mL
10 mM0.5045 mL2.5224 mL5.0449 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: 10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (12.61 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (12.61 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液
  • 2.

    请依序添加每种溶剂: 10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (12.61 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (12.61 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
  • 3.

    请依序添加每种溶剂: 10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (12.61 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (12.61 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在 MCE 网站选购。
Purity ≥98.0%

Information

Intro o-Phenanthroline monohydrate (1,10-Phenanthroline monohydrate) 是一种金属螯合剂,可防止链脲佐菌素诱导染色体畸变。o-Phenanthroline monohydrate 与 Fe2+ 形成红色螯合物,在 510 nm 处最大吸收。o-Phenanthroline (1,10-Phenanthroline) monohydrate 也是 MMP 的抑制剂。
Synonym 1,10-Phenanthroline monohydrate
Rela_targets Natural Product-like Compound Library Bioactive Compound Library Plus Metabolism/Protease Compound Library Fragment Library Differentiation Inducing Compound Library FDA Approved & Pharmacopeial Drug Library Anti-Pancreatic Cancer Compound Library Angiogenesis-Related Compound Library Human Metabolite Library Anti-Pulmonary Fibrosis Compound Library Heterocyclic Compound Library Pain-Related Compound Library Membrane Protein-targeted Compound Library
AYB26723