iNOS (YD36290) Rabbit mAb (Cat.#:AYD16613)
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Cat.#
AYD16613
Target / gene
NOS2/Nos2
Host
Rabbit
Clonality
Monoclonal
Reactivity
Human, Mouse, Rat
Application
IHC-P, FC
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Cat.#：AYD16613
| Size | Price | |
|---|---|---|
| 50ul | RMB: 680Sale1280 | Add to cart |
| 100ul | RMB: 1219Sale2300 | Add to cart |
Sales Terms | Request COA | Stock and shipping are confirmed by support
Information
Application guide
Related products
Immunogen
Target data
Resources
FAQs
| Reactivity | Human, Mouse, Rat |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Application | IHC-PFC |
| Dilution | |
| Predicted MW | 131kDa/131kDa/131kDa |
| Observed MW | |
| Format | Liquid |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.75% BSA,50% glycerol,pH7.3. |
| Conjugate | Unconjugated |
| Positive | RAW264.7 |
| Subcellular | Cytoplasm, cytosol |
| Purification | 亲和纯化 |
Applications and conditions
Where to start
These starting conditions come from the recommended dilution, validation images, and general assay practice. Titrate for your sample and detection system.
IHC-P
Conditions for IHC-P
| Recommended dilution | See the validation images or contact technical support |
|---|---|
| Suggested sample / positive control | RAW264.7 |
| Key conditions | For paraffin sections, optimize retrieval buffer pH, retrieval time, and primary incubation |
| Expected result | Expected localization: Cytoplasm, cytosol |
| Controls | Include a positive sample, a negative sample, and a secondary or isotype control |
FC
Conditions for FC
| Recommended dilution | See the validation images or contact technical support |
|---|---|
| Suggested sample / positive control | RAW264.7 |
| Key conditions | For intracellular targets, optimize fixation and permeabilization, and include an isotype control |
| Expected result | Expect the positive population to shift relative to the negative or isotype control |
| Controls | Include a positive sample, a negative sample, and a secondary or isotype control |
Related products
More associated products >>
Immunogen
| Immunogen | Please contact Support |
|---|---|
| Sequence | Request sequence |
Target data
| Background | Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body (PubMed:7504305, PubMed:7531687, PubMed:7544004, PubMed:7682706). In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2 (By similarity). As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH on 'Cys-247' implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM (PubMed:25417112). Involved in inflammation, enhances the synthesis of pro-inflammatory mediators such as IL6 and IL8 (PubMed:19688109) Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body (PubMed:7503239). In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2 (PubMed:16373578). As component of the iNOS-S100A8/9 transnitrosylase complex is involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM (By similarity). Involved in inflammation, enhances the synthesis of pro-inflammatory mediators such as IL6 and IL8 (By similarity) Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2. As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM. Involved in inflammation, enhances the synthesis of pro-inflammatory mediators such as IL6 and IL8 |
|---|---|
| Gene ID | 4843 |
| Gene name | NOS2, Nos2 |
| Swiss | P35228, P29477, Q06518 |
| Synonym | iNOS (YD36290),iNOS (YD36290) Rabbit mAb,NOS2,Hepatocyte NOS,Inducible NO synthase,NOS type II,Peptidyl-cysteine S-nitrosylase NOS2,Macrophage NOS,NOS2A,Inosl |
Resources
Validation data
Contact technical support for validation images and application examples.
References
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Send feedbackSupport
Support can help confirm the application, sample, dilution, controls, and assay conditions.
Support center
Need help with the application?
Send the catalog number, application, sample type, and assay. Support can confirm dilution, controls, and COA or lot documents.
FAQs
Listed applications: IHC-P, FC. Use the validation images and recommended dilution as a starting point.
Send the catalog number and lot through the inquiry form. Support will help with the lot documents.
Use the recommended conditions as a starting concentration. Sample, fixation, and detection system may need a titration.