ZO-1 (YD20011) Rabbit mAb (Cat.#:AYD16102)
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Cat.#：AYD16102
| Size | Price | |
|---|---|---|
| 50ul | RMB: 680Sale1280 | Add to cart |
| 100ul | RMB: 1219Sale2300 | Add to cart |
Sales Terms | Request COA | Stock and shipping are confirmed by support
Information
Application guide
Related products
Immunogen
Target data
Resources
FAQs
| Reactivity | Human,Mouse,Rat |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Application | IHC-PICC/IFFC |
| Dilution | |
| Predicted MW | 195kDa |
| Observed MW | |
| Format | Liquid |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.75% BSA,50% glycerol,pH7.3. |
| Conjugate | Unconjugated |
| Positive | HeLa |
| Subcellular | Cell membrane, Cell junction, tight junction, gap junction, Cell projection, podosome |
| Purification | 亲和纯化 |
Applications and conditions
These starting conditions come from the recommended dilution, validation images, and general assay practice. Titrate for your sample and detection system.
| Recommended dilution | See the validation images or contact technical support |
|---|---|
| Suggested sample / positive control | HeLa |
| Key conditions | For paraffin sections, optimize retrieval buffer pH, retrieval time, and primary incubation |
| Expected result | Expected localization: Cell membrane, Cell junction, tight junction, gap junction, Cell projection, podosome |
| Controls | Include a positive sample, a negative sample, and a secondary or isotype control |
| Recommended dilution | See the validation images or contact technical support |
|---|---|
| Suggested sample / positive control | HeLa |
| Key conditions | Optimize fixation, permeabilization, and blocking, and include a secondary-only control |
| Expected result | Expected fluorescence localization: Cell membrane, Cell junction, tight junction, gap junction, Cell projection, podosome |
| Controls | Include a positive sample, a negative sample, and a secondary or isotype control |
| Recommended dilution | See the validation images or contact technical support |
|---|---|
| Suggested sample / positive control | HeLa |
| Key conditions | For intracellular targets, optimize fixation and permeabilization, and include an isotype control |
| Expected result | Expect the positive population to shift relative to the negative or isotype control |
| Controls | Include a positive sample, a negative sample, and a secondary or isotype control |
Related products
More associated products >>
Immunogen
| Immunogen | Please contact Support |
|---|---|
| Sequence | Request sequence |
Target data
| Background | This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. Alternative splicing of this gene results in multiple transcript variants. |
|---|---|
| Gene ID | 7082 |
| Gene name | TJP1 |
| Swiss | Q07157 |
| Synonym | ZO-1 (YD20011),ZO-1 (YD20011) Rabbit mAb,TJP1,Tight junction protein ZO-1,Zona occludens protein 1,Zonula occludens protein 1,ZO1 |
Resources
Validation data
1 images
Validation images at the top of the page cover IHC-P / ICC/IF / FC.
References
No papers are linked yet. Contact technical support for application examples.
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