0
Home Products Antibodies Primary antibodies Secondary antibodies Tag antibodies Loading control antibodies Premium polyclonal antibodies Recombinant rabbit mAbs Mouse mAbs Phosphorylated antibodies Methylation antibodies Acetylated antibodies Biochemicals Research Areas
Service & Support Custom Services Support Promotion About Us
Sign In Sign Up

VEGF Receptor 2 (YD20041) Rabbit mAb  (Cat.#:AYD12825)

A
小A Scan to ask
Host: RabbitClonality: MonoclonalReactivity: MouseICC/IFFC
Datasheet    Copy details    Copy citation
Cat.# AYD12825
Target / gene Kdr
Host Rabbit
Clonality Monoclonal
Reactivity Mouse
Application ICC/IF, FC

暂无验证图

欢迎提交实验图片，或联系 Support 获取更多信息。

Cat.#：AYD12825

SizePrice
50ul RMB: 680Sale1280 Add to cart
100ul RMB: 1219Sale2300 Add to cart

Promotion  |  Support

Sales Terms  |  Request COA  |  Stock and shipping are confirmed by support

  • Information

  • Application guide

  • Related products

  • Immunogen

  • Target data

  • Resources

  • FAQs

Reactivity Mouse
Host Rabbit
Clonality Monoclonal
Isotype IgG
Application ICC/IFFC
Dilution
Predicted MW 150kDa
Observed MW
Format Liquid
Storage Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.75% BSA,50% glycerol,pH7.3.
Conjugate Unconjugated
Positive Heart,Lung,Kidney
Subcellular Cell junction, Endoplasmic reticulum, Cell membrane, Cytoplasm, Nucleus, Cytoplasmic vesicle, Early endosome, Secreted
Purification 亲和纯化

Applications and conditions

Where to start

These starting conditions come from the recommended dilution, validation images, and general assay practice. Titrate for your sample and detection system.

ICC/IF Conditions for ICC/IF
Recommended dilution See the validation images or contact technical support
Suggested sample / positive control Heart,Lung,Kidney
Key conditions Optimize fixation, permeabilization, and blocking, and include a secondary-only control
Expected result Expected fluorescence localization: Cell junction, Endoplasmic reticulum, Cell membrane, Cytoplasm, Nucleus, Cytoplasmic vesicle, Early endosome, Secreted
Controls Include a positive sample, a negative sample, and a secondary or isotype control
FC Conditions for FC
Recommended dilution See the validation images or contact technical support
Suggested sample / positive control Heart,Lung,Kidney
Key conditions For intracellular targets, optimize fixation and permeabilization, and include an isotype control
Expected result Expect the positive population to shift relative to the negative or isotype control
Controls Include a positive sample, a negative sample, and a secondary or isotype control

Related products

More associated products >>
VEGF Receptor 2 (YD15717) Rabbit mAb
Same target
AYD15008
VEGF Receptor 2 (YD15717) Rabbit mAb
WB

Immunogen

Immunogen Please contact Support
Sequence Request sequence

Target data

Background Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFC and VEGFD. Plays an essential role in the regulation of angiogenesis, vascular development, vascular permeability, and embryonic hematopoiesis. Promotes proliferation, survival, migration and differentiation of endothelial cells. Promotes reorganization of the actin cytoskeleton. Isoforms lacking a transmembrane domain, such as isoform 2, may function as decoy receptors for VEGFA, VEGFC and/or VEGFD. Isoform 2 plays an important role as a negative regulator of VEGFA- and VEGFC-mediated lymphangiogenesis by limiting the amount of free VEGFA and/or VEGFC and by preventing their binding to FLT4. Modulates FLT1 and FLT4 signaling by forming heterodimers. Binding of vascular growth factors to isoform 1 leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate and the activation of protein kinase C. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, reorganization of the actin cytoskeleton and activation of PTK2/FAK1. Required for VEGFA-mediated induction of NOS2 and NOS3, leading to the production of the signaling molecule nitric oxide (NO) by endothelial cells. Phosphorylates PLCG1. Promotes phosphorylation of FYN, NCK1, NOS3, PIK3R1, PTK2/FAK1 and SRC
Gene ID 3134
Gene name Kdr
Swiss P35918
Synonym VEGF Receptor 2 (YD20041),VEGF Receptor 2 (YD20041) Rabbit mAb,Kdr,Fetal liver kinase 1,Kinase NYK,Protein-tyrosine kinase receptor flk-1,Flk-1,Flk1

Resources

Validation data

Contact technical support for validation images and application examples.

References

No papers are linked yet. Contact technical support for application examples.

Ask for examples
Customer reviews

No reviews yet. After use, you can submit a review and experiment images.

Send feedback
Support

Support can help confirm the application, sample, dilution, controls, and assay conditions.

Support center
Need help with the application?

Send the catalog number, application, sample type, and assay. Support can confirm dilution, controls, and COA or lot documents.

Support Request COA

FAQs

Listed applications: ICC/IF, FC. Use the validation images and recommended dilution as a starting point.
Send the catalog number and lot through the inquiry form. Support will help with the lot documents.
Use the recommended conditions as a starting concentration. Sample, fixation, and detection system may need a titration.
AYD12825