VEGFA (YD15508) Rabbit mAb (Cat.#:AYD15046)
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Cat.#
AYD15046
Target / gene
VEGFA/Vegfa
Host
Rabbit
Clonality
Monoclonal
Reactivity
Human, Mouse
Application
IHC-P, ICC/IF, FC
Cat.#：AYD15046
| Size | Price | |
|---|---|---|
| 50ul | RMB: 680Sale1280 | Add to cart |
| 100ul | RMB: 1219Sale2300 | Add to cart |
Sales Terms | Request COA | Stock and shipping are confirmed by support
Information
Application guide
Related products
Immunogen
Target data
Resources
FAQs
| Reactivity | Human,Mouse |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Application | IHC-PICC/IFFC |
| Dilution | |
| Predicted MW | 44kDa/43kDa |
| Observed MW | |
| Format | Liquid |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.75% BSA,50% glycerol,pH7.3. |
| Conjugate | Unconjugated |
| Positive | K-562,U-87MG,HeLa,Mouse brain,Rat brain |
| Subcellular | Cytoplasm, Nucleus, Secreted, Endoplasmic reticulum, Golgi apparatus, extracellular space, extracellular matrix, Cell membrane |
| Purification | 亲和纯化 |
Applications and conditions
Where to start
These starting conditions come from the recommended dilution, validation images, and general assay practice. Titrate for your sample and detection system.
IHC-P
Conditions for IHC-P
| Recommended dilution | See the validation images or contact technical support |
|---|---|
| Suggested sample / positive control | K-562,U-87MG,HeLa,Mouse brain,Rat brain |
| Key conditions | For paraffin sections, optimize retrieval buffer pH, retrieval time, and primary incubation |
| Expected result | Expected localization: Cytoplasm, Nucleus, Secreted, Endoplasmic reticulum, Golgi apparatus, extracellular space, extracellular matrix, Cell membrane |
| Controls | Include a positive sample, a negative sample, and a secondary or isotype control |
ICC/IF
Conditions for ICC/IF
| Recommended dilution | See the validation images or contact technical support |
|---|---|
| Suggested sample / positive control | K-562,U-87MG,HeLa,Mouse brain,Rat brain |
| Key conditions | Optimize fixation, permeabilization, and blocking, and include a secondary-only control |
| Expected result | Expected fluorescence localization: Cytoplasm, Nucleus, Secreted, Endoplasmic reticulum, Golgi apparatus, extracellular space, extracellular matrix, Cell membrane |
| Controls | Include a positive sample, a negative sample, and a secondary or isotype control |
FC
Conditions for FC
| Recommended dilution | See the validation images or contact technical support |
|---|---|
| Suggested sample / positive control | K-562,U-87MG,HeLa,Mouse brain,Rat brain |
| Key conditions | For intracellular targets, optimize fixation and permeabilization, and include an isotype control |
| Expected result | Expect the positive population to shift relative to the negative or isotype control |
| Controls | Include a positive sample, a negative sample, and a secondary or isotype control |
Related products
More associated products >>
Immunogen
| Immunogen | Please contact Support |
|---|---|
| Sequence | Request sequence |
Target data
| Background | Participates in the induction of key genes involved in the response to hypoxia and in the induction of angiogenesis such as HIF1A (PubMed:35455969). Involved in protecting cells from hypoxia-mediated cell death (By similarity) Participates in the induction of key genes involved in the response to hypoxia and in the induction of angiogenesis such as HIF1A (PubMed:35455969). Involved in protecting cells from hypoxia-mediated cell death (PubMed:35455969) |
|---|---|
| Gene ID | 7422 |
| Gene name | VEGFA, Vegfa |
| Swiss | P15692, Q00731 |
| Synonym | VEGFA (YD15508),VEGFA (YD15508) Rabbit mAb,VEGFA,Vascular permeability factor,VEGF |
Resources
Validation data
1 images
Validation images at the top of the page cover IHC-P / ICC/IF / FC.
References
No papers are linked yet. Contact technical support for application examples.
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Send the catalog number, application, sample type, and assay. Support can confirm dilution, controls, and COA or lot documents.
FAQs
Listed applications: IHC-P, ICC/IF, FC. Use the validation images and recommended dilution as a starting point.
Send the catalog number and lot through the inquiry form. Support will help with the lot documents.
Use the recommended conditions as a starting concentration. Sample, fixation, and detection system may need a titration.