TREM2 (YD33097) Rabbit mAb (Cat.#:AYD16644)
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Cat.#
AYD16644
Target / gene
Trem2
Host
Rabbit
Clonality
Monoclonal
Reactivity
Mouse
Application
ICC/IF, FC
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Cat.#：AYD16644
| Size | Price | |
|---|---|---|
| 50ul | RMB: 680Sale1280 | Add to cart |
| 100ul | RMB: 1219Sale2300 | Add to cart |
Sales Terms | Request COA | Stock and shipping are confirmed by support
Information
Application guide
Immunogen
Target data
Resources
FAQs
| Reactivity | Mouse |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Application | ICC/IFFC |
| Dilution | |
| Predicted MW | 25kDa |
| Observed MW | |
| Format | Liquid |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.75% BSA,50% glycerol,pH7.3. |
| Conjugate | Unconjugated |
| Positive | Brain |
| Subcellular | Cell membrane, Secreted |
| Purification | 亲和纯化 |
Applications and conditions
Where to start
These starting conditions come from the recommended dilution, validation images, and general assay practice. Titrate for your sample and detection system.
ICC/IF
Conditions for ICC/IF
| Recommended dilution | See the validation images or contact technical support |
|---|---|
| Suggested sample / positive control | Brain |
| Key conditions | Optimize fixation, permeabilization, and blocking, and include a secondary-only control |
| Expected result | Expected fluorescence localization: Cell membrane, Secreted |
| Controls | Include a positive sample, a negative sample, and a secondary or isotype control |
FC
Conditions for FC
| Recommended dilution | See the validation images or contact technical support |
|---|---|
| Suggested sample / positive control | Brain |
| Key conditions | For intracellular targets, optimize fixation and permeabilization, and include an isotype control |
| Expected result | Expect the positive population to shift relative to the negative or isotype control |
| Controls | Include a positive sample, a negative sample, and a secondary or isotype control |
Immunogen
| Immunogen | Please contact Support |
|---|---|
| Sequence | Request sequence |
Target data
| Background | Forms a receptor signaling complex with TYROBP which mediates signaling and cell activation following ligand binding (PubMed:11241283). Acts as a receptor for amyloid-beta protein 42, a cleavage product of the amyloid-beta precursor protein APP, and mediates its uptake and degradation by microglia (PubMed:27477018, PubMed:29518356). Binding to amyloid-beta 42 mediates microglial activation, proliferation, migration, apoptosis and expression of pro-inflammatory cytokines, such as IL6R and CCL3, and the anti-inflammatory cytokine ARG1 (PubMed:27477018, PubMed:29518356). Acts as a receptor for lipoprotein particles such as LDL, VLDL, and HDL and for apolipoproteins such as APOA1, APOA2, APOB, APOE, APOE2, APOE3, APOE4, and CLU and enhances their uptake in microglia (PubMed:27477018). Binds phospholipids (preferably anionic lipids) such as phosphatidylserine, phosphatidylethanolamine, phosphatidylglycerol and sphingomyelin (By similarity). Regulates microglial proliferation by acting as an upstream regulator of the Wnt/beta-catenin signaling cascade (PubMed:28077724). Required for microglial phagocytosis of apoptotic neurons (PubMed:24990881). Also required for microglial activation and phagocytosis of myelin debris after neuronal injury and of neuronal synapses during synapse elimination in the developing brain (PubMed:15728241, PubMed:25631124, PubMed:28592261, PubMed:29752066). Regulates microglial chemotaxis and process outgrowth, and also the microglial response to oxidative stress and lipopolysaccharide (PubMed:28483841, PubMed:29663649, PubMed:29859094, PubMed:30232263). It suppresses PI3K and NF-kappa-B signaling in response to lipopolysaccharide; thus promoting phagocytosis, suppressing pro-inflammatory cytokine and nitric oxide production, inhibiting apoptosis and increasing expression of IL10 and TGFB (PubMed:29663649). During oxidative stress, it promotes anti-apoptotic NF-kappa-B signaling and ERK signaling (PubMed:28592261). Plays a role in microglial MTOR activation and metabolism (PubMed:28802038). Regulates age-related changes in microglial numbers (PubMed:25631124, PubMed:29752066, PubMed:30548312). Triggers activation of the immune responses in macrophages and dendritic cells. Mediates cytokine-induced formation of multinucleated giant cells which are formed by the fusion of macrophages (PubMed:18957693). In dendritic cells, receptor of SEMA6D with PLEXNA1 as coreceptor and mediates up-regulation of chemokine receptor CCR7 and dendritic cell maturation and survival (PubMed:16715077). Involved in the positive regulation of osteoclast differentiation (PubMed:16418779) |
|---|---|
| Gene ID | 3134 |
| Gene name | Trem2 |
| Swiss | Q99NH8 |
| Synonym | TREM2 (YD33097),TREM2 (YD33097) Rabbit mAb,Trem2,Triggering receptor expressed on monocytes 2,Trem2a,Trem2b,Trem2c |
Resources
Validation data
Contact technical support for validation images and application examples.
References
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FAQs
Listed applications: ICC/IF, FC. Use the validation images and recommended dilution as a starting point.
Send the catalog number and lot through the inquiry form. Support will help with the lot documents.
Use the recommended conditions as a starting concentration. Sample, fixation, and detection system may need a titration.