Recombinant Proteinase K (Cat.#:AYB26860)
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Biological activity
Information
Biological activity
| Bioactivity | Recombinant Proteinase K is a serine protease that cleaves the carboxy-terminated peptide bonds of aliphatic and aromatic amino acids. Recombinant Proteinase K can be used to digest proteins and remove contamination from nucleic acid preparations. |
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| Chinese name | 重组蛋白酶 K |
| In vitro | Proteinase K digestion1. Cells are resuspended in lysis bufferand finally lysed on ice for 15 minutes.2. The crude lysates are obtained by centrifugation at 4°C for 15 minutes. Proteinase K is added to a 20 µl aliquot to get the final concentrations of 2, 4 and 8 µg/ml. Reactions are carried out at 37°C for 30 minutes. The accuracy of these methods have not been independently confirmed. They are for reference only. |
| Format | Solid |
| Transportation | Room temperature in continental US; may vary elsewhere. |
| Storage | -20°C, sealed storage, away from moisture |
Information
| Intro | Recombinant Proteinase K 是一种丝氨酸蛋白酶 (serine protease)，它能裂解脂肪族和芳香族氨基酸的羧基端肽键。Recombinant Proteinase K 可用于消化蛋白质和清除核酸制剂中的污染。 |
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| Synonym | 重组蛋白酶 K |