Photo-lysine hydrochloride (Cat.#:AYB26845)
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Biological activity
Information
Biological activity
| Bioactivity | Photo-lysine hydrochloride, a new lysine-based photo-reactive amino acid, captures proteins that bind lysine post-translational modifications. | ||||||||||||||||
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| Mol.Wt. | 245.11 | ||||||||||||||||
| In vitro | Photo-lysine is designed and synthesized by incorporating a photo-cross-linker (diazirine) into the side chain of natural lysine. Photo-lysine, which is readily incorporated into proteins by native mammalian translation machinery, can be used to capture and identify proteins that recognize lysine post-translational modifications (PTMs), including ‘readers’ and ‘erasers’ of histone modifications. Photo-lysine can be incorporated into MDH2 and mediate photo-cross-linking to fix protein-protein interactions in cells. UV irradiation of cells in the presence of photo-lysine induced robust cross-linking of HSP90β and HSP60. Photo-lysine has higher efficiency than photo-leucine for photo-cross-linking of the two chaperone proteins. Photo-lysine enables capture of the heterodimer of proteins Ku70 and Ku80 within a protein complex. Photo-lysine enables identification of histone- and chromatin-binding proteins. The accuracy of these methods have not been independently confirmed. They are for reference only. |
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| Format | Solid | ||||||||||||||||
| Transportation | Room temperature in continental US; may vary elsewhere. | ||||||||||||||||
| Storage | 4°C, protect from light, stored under nitrogen | ||||||||||||||||
| Solubility | In Vitro: H2O : ≥ 40 mg/mL (163.19 mM) *"≥" means soluble, but saturation unknown. 配制储备液
* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 |
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| Purity | ≥98.0% |
Information
| Intro | Photo-lysine hydrochloride，一种新的基于赖氨酸光反应性的氨基酸，它捕获结合赖氨酸翻译后修饰的蛋白质。 |
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