PCNT (YD16098) Rabbit mAb (Cat.#:AYD16757)
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Cat.#：AYD16757
| Size | Price | |
|---|---|---|
| 50ul | RMB: 680Sale1280 | Add to cart |
| 100ul | RMB: 1219Sale2300 | Add to cart |
Sales Terms | Request COA | Stock and shipping are confirmed by support
Information
Application guide
Related products
Immunogen
Target data
Resources
FAQs
| Reactivity | Human, Mouse |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Application | IHC-PICC/IFFC |
| Dilution | |
| Predicted MW | 378kDa |
| Observed MW | |
| Format | Liquid |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.75% BSA,50% glycerol,pH7.3. |
| Conjugate | Unconjugated |
| Positive | SH-SY5Y,Rat heart |
| Subcellular | Cytoplasm, cytoskeleton, microtubule organizing center, centrosome |
| Purification | 亲和纯化 |
Applications and conditions
These starting conditions come from the recommended dilution, validation images, and general assay practice. Titrate for your sample and detection system.
| Recommended dilution | See the validation images or contact technical support |
|---|---|
| Suggested sample / positive control | SH-SY5Y,Rat heart |
| Key conditions | For paraffin sections, optimize retrieval buffer pH, retrieval time, and primary incubation |
| Expected result | Expected localization: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome |
| Controls | Include a positive sample, a negative sample, and a secondary or isotype control |
| Recommended dilution | See the validation images or contact technical support |
|---|---|
| Suggested sample / positive control | SH-SY5Y,Rat heart |
| Key conditions | Optimize fixation, permeabilization, and blocking, and include a secondary-only control |
| Expected result | Expected fluorescence localization: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome |
| Controls | Include a positive sample, a negative sample, and a secondary or isotype control |
| Recommended dilution | See the validation images or contact technical support |
|---|---|
| Suggested sample / positive control | SH-SY5Y,Rat heart |
| Key conditions | For intracellular targets, optimize fixation and permeabilization, and include an isotype control |
| Expected result | Expect the positive population to shift relative to the negative or isotype control |
| Controls | Include a positive sample, a negative sample, and a secondary or isotype control |
Related products
More associated products >>
Immunogen
| Immunogen | Please contact Support |
|---|---|
| Sequence | Request sequence |
Target data
| Background | The protein encoded by this gene binds to calmodulin and is expressed in the centrosome. It is an integral component of the pericentriolar material (PCM). The protein contains a series of coiled-coil domains and a highly conserved PCM targeting motif called the PACT domain near its C-terminus. The protein interacts with the microtubule nucleation component gamma-tubulin and is likely important to normal functioning of the centrosomes, cytoskeleton, and cell-cycle progression. Mutations in this gene cause Seckel syndrome-4 and microcephalic osteodysplastic primordial dwarfism type II. Two transcript variants encoding different isoforms have been found for this gene. |
|---|---|
| Gene ID | 5116 |
| Gene name | PCNT |
| Swiss | O95613 |
| Synonym | PCNT (YD16098),PCNT (YD16098) Rabbit mAb,PCNT,Kendrin,Pericentrin-B,KIAA0402,PCNT2 |
Resources
Validation data
Contact technical support for validation images and application examples.
References
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