NG2 (YD35404) Rabbit mAb (Cat.#:AYD16149)
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Cat.#
AYD16149
Target / gene
Cspg4
Host
Rabbit
Clonality
Monoclonal
Reactivity
Mouse, Rat
Application
IHC-P, ICC/IF, FC
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Cat.#：AYD16149
| Size | Price | |
|---|---|---|
| 50ul | RMB: 680Sale1280 | Add to cart |
| 100ul | RMB: 1219Sale2300 | Add to cart |
Sales Terms | Request COA | Stock and shipping are confirmed by support
Information
Application guide
Related products
Immunogen
Target data
Resources
FAQs
| Reactivity | Mouse,Rat |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Application | IHC-PICC/IFFC |
| Dilution | |
| Predicted MW | 252kDa/252kDa |
| Observed MW | |
| Format | Liquid |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.75% BSA,50% glycerol,pH7.3. |
| Conjugate | Unconjugated |
| Positive | A375,Mouse lung |
| Subcellular | Cell membrane, Apical cell membrane, Cell projection, lamellipodium membrane, Cell surface |
| Purification | 亲和纯化 |
Applications and conditions
Where to start
These starting conditions come from the recommended dilution, validation images, and general assay practice. Titrate for your sample and detection system.
IHC-P
Conditions for IHC-P
| Recommended dilution | See the validation images or contact technical support |
|---|---|
| Suggested sample / positive control | A375,Mouse lung |
| Key conditions | For paraffin sections, optimize retrieval buffer pH, retrieval time, and primary incubation |
| Expected result | Expected localization: Cell membrane, Apical cell membrane, Cell projection, lamellipodium membrane, Cell surface |
| Controls | Include a positive sample, a negative sample, and a secondary or isotype control |
ICC/IF
Conditions for ICC/IF
| Recommended dilution | See the validation images or contact technical support |
|---|---|
| Suggested sample / positive control | A375,Mouse lung |
| Key conditions | Optimize fixation, permeabilization, and blocking, and include a secondary-only control |
| Expected result | Expected fluorescence localization: Cell membrane, Apical cell membrane, Cell projection, lamellipodium membrane, Cell surface |
| Controls | Include a positive sample, a negative sample, and a secondary or isotype control |
FC
Conditions for FC
| Recommended dilution | See the validation images or contact technical support |
|---|---|
| Suggested sample / positive control | A375,Mouse lung |
| Key conditions | For intracellular targets, optimize fixation and permeabilization, and include an isotype control |
| Expected result | Expect the positive population to shift relative to the negative or isotype control |
| Controls | Include a positive sample, a negative sample, and a secondary or isotype control |
Related products
More associated products >>
Immunogen
| Immunogen | Please contact Support |
|---|---|
| Sequence | Request sequence |
Target data
| Background | Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N-terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Also functions as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N-terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Also functions as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades |
|---|---|
| Gene ID | 3134 |
| Gene name | Cspg4 |
| Swiss | Q8VHY0, Q00657 |
| Synonym | NG2 (YD35404),NG2 (YD35404) Rabbit mAb,Cspg4,Chondroitin sulfate proteoglycan NG2,Proteoglycan AN2,HSN tumor-specific antigen,An2,Kiaa4232,Ng2 |
Resources
Validation data
Contact technical support for validation images and application examples.
References
No papers are linked yet. Contact technical support for application examples.
Ask for examplesCustomer reviews
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Send feedbackSupport
Support can help confirm the application, sample, dilution, controls, and assay conditions.
Support center
Need help with the application?
Send the catalog number, application, sample type, and assay. Support can confirm dilution, controls, and COA or lot documents.
FAQs
Listed applications: IHC-P, ICC/IF, FC. Use the validation images and recommended dilution as a starting point.
Send the catalog number and lot through the inquiry form. Support will help with the lot documents.
Use the recommended conditions as a starting concentration. Sample, fixation, and detection system may need a titration.