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N6-methyladenosine (m6A) Rabbit mAb  (Cat.#:AAY1655)

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Host: RabbitClonality: MonoclonalReactivity: Human,Mouse,RatDot blot
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Cat.# AAY1655
Target / gene N6-methyladenosine (m6A) Rabbit mAb
Host Rabbit
Clonality Monoclonal
Reactivity Human, Mouse, Rat
Application Dot blot

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Cat.#：AAY1655

SizePrice
50ul ￥1150.00 Add to cart
100ul ￥2100.00 Add to cart

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  • Information

  • Application guide

  • Immunogen

  • Target data

  • Resources

  • FAQs

Reactivity Human,Mouse,Rat
Host Rabbit
Clonality Monoclonal
Clone no. AA0889R
Isotype IgG,Kappa
Application Dot blot
Dilution DB: 1:500-1:2000
Predicted MW
Observed MW
Format Liquid
Storage Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.75% BSA,50% glycerol,pH7.657.
Conjugate Unconjugated
Positive Arabidopsis
Subcellular Cell membrane,Cytoplasm
Purification 亲和纯化

Applications and conditions

Where to start

These starting conditions come from the recommended dilution, validation images, and general assay practice. Titrate for your sample and detection system.

Dot blot Conditions for Dot blot
Recommended dilution See the validation images or contact technical support
Suggested sample / positive control Arabidopsis
Key conditions Start at the recommended dilution and titrate for your sample and assay
Expected result Expected signal follows target localization and the validation images
Controls Include a positive sample, a negative sample, and a secondary or isotype control

Immunogen

Immunogen Please contact Support
Sequence Request sequence

Target data

Background Discovered in the 1970s, m6A is the most prevalent internal modification in polyadenylated mRNAs and long non-coding RNAs (lncRNAs) in higher eukaryotes. m6A is widely conserved among eukaryotic species that range from yeast, plants, flies to mammals, as well as among viral RNAs with a nuclear phase. The m6A-based modification is associated with a well-defined RNA motif, RRACH (R: A/G, H: A/C/U). As a representative of the epitranscriptome, m6A mRNA modifications participate in many vital activities in the cell, including stem cell self-renewal and differentiation, mRNA transcription, alternative splicing, nuclear export, translation, degradation, and microRNA processing. These processes determine the expression or inactivation of specific genes, which is vital for growth and development.(PMID: 30416848; PMID: 24662220; PMID: 30429466)
Gene ID
Gene name
Swiss
Synonym ;;

Resources

Validation data

Contact technical support for validation images and application examples.

References

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FAQs

Listed applications: Dot blot. Use the validation images and recommended dilution as a starting point.
Send the catalog number and lot through the inquiry form. Support will help with the lot documents.
Use the recommended conditions as a starting concentration. Sample, fixation, and detection system may need a titration.
AAY1655