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Methylproamine  (Cat.#:AYB26809)

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Cat.#:AYB26809

CAS:188247-01-0

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50 mg ¥2400.00 AddToCart
10 mM * 1 mL in DMSO ¥2640.00 AddToCart
100 mg ¥3200.00 AddToCart
  • Biological activity

  • Information

Biological activity

Bioactivity Methylproamine is a DNA-binding radioprotector, acts by repair of transient radiation-induced oxidative species on DNA. Methylproamine also protects against ionizing radiation by preventing DNA double-strand breaks.
CAS 188247-01-0
Mol.Wt. 465.59
In vitro

Methylproamine also protects against ionizing radiation by preventing DNA double-strand breaks.
Methylproamine can protect bystander cells from radiation-induced DNA damage.
Methylproamine has a concentration-dependent radioprotective effect

The accuracy of these methods have not been independently confirmed. They are for reference only.

Cell Cytotoxicity Assay

Cell Line:Keratinocytes
Concentration:10, 20 μM
Incubation Time:60 min
Result:Did not show any detectable cytotoxicity at 10 μM and had appreciable cytotoxicity at 20 μM.
Format Solid
Transportation Room temperature in continental US; may vary elsewhere.
Solubility
In Vitro: 

DMSO : ≥ 41 mg/mL (88.06 mM)

*"≥" means soluble, but saturation unknown.

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM2.1478 mL10.7391 mL21.4781 mL
5 mM0.4296 mL2.1478 mL4.2956 mL
10 mM0.2148 mL1.0739 mL2.1478 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: 10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 0.62 mg/mL (1.33 mM); Clear solution

    此方案可获得 ≥ 0.62 mg/mL (1.33 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 6.2 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液
  • 2.

    请依序添加每种溶剂: 10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 0.62 mg/mL (1.33 mM); Clear solution

    此方案可获得 ≥ 0.62 mg/mL (1.33 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 6.2 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
*以上所有助溶剂都可在 MCE 网站选购。
Purity 99.90%

Information

Intro Methylproamine 是 DNA结合的辐射保护剂,它通过修复短暂辐射诱导的 DNA 氧化物种起作用,也可通过保护 DNA 双链断裂来防止电离辐射。
Rela_targets Bioactive Compound Library Plus Cell Cycle/DNA Damage Compound Library Anti-Aging Compound Library
AYB26809