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MCM3 (YD11254) Rabbit mAb  (Cat.#:AYD11512)

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Host: RabbitClonality: MonoclonalReactivity: HumanIHC-P
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Cat.# AYD11512
Target / gene MCM3
Host Rabbit
Clonality Monoclonal
Reactivity Human
Application IHC-P

Cat.#：AYD11512

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50ul RMB: 680Sale1280 Add to cart
100ul RMB: 1219Sale2300 Add to cart

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  • Information

  • Application guide

  • Related products

  • Immunogen

  • Target data

  • Resources

  • FAQs

Reactivity Human
Host Rabbit
Clonality Monoclonal
Isotype IgG
Application IHC-P
Dilution
Predicted MW 91kDa
Observed MW
Format Liquid
Storage Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.75% BSA,50% glycerol,pH7.3.
Conjugate Unconjugated
Positive HeLa,293T,PC-12,NIH/3T3,COS-1,COS-7
Subcellular Nucleus, Chromosome
Purification 亲和纯化

Applications and conditions

Where to start

These starting conditions come from the recommended dilution, validation images, and general assay practice. Titrate for your sample and detection system.

IHC-P Conditions for IHC-P
Recommended dilution See the validation images or contact technical support
Suggested sample / positive control HeLa,293T,PC-12,NIH/3T3,COS-1,COS-7
Key conditions For paraffin sections, optimize retrieval buffer pH, retrieval time, and primary incubation
Expected result Expected localization: Nucleus, Chromosome
Controls Include a positive sample, a negative sample, and a secondary or isotype control

Related products

More associated products >>
MCM3 Rabbit mAb
Same target
AYM29434
MCM3 Rabbit mAb
WB,IHC,IF/ICC,FC

Immunogen

Immunogen Please contact Support
Sequence Request sequence

Target data

Background The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with and is acetylated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. Two transcript variants encoding different isoforms have been found for this gene.
Gene ID 4172
Gene name MCM3
Swiss P25205
Synonym MCM3 (YD11254),MCM3 (YD11254) Rabbit mAb,MCM3,DNA polymerase alpha holoenzyme-associated protein P1,P1-MCM3,RLF subunit beta,p102

Resources

Validation data

2 images

Validation images at the top of the page cover IHC-P.

References

No papers are linked yet. Contact technical support for application examples.

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FAQs

Listed applications: IHC-P. Use the validation images and recommended dilution as a starting point.
Send the catalog number and lot through the inquiry form. Support will help with the lot documents.
Use the recommended conditions as a starting concentration. Sample, fixation, and detection system may need a titration.
AYD11512