HEPES (Cat.#:AYB26681)

Name: HEPES
Brand: 艾博立生物
SKU: AYB26681
Availability: InStock

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Cat.#：AYB26681

CAS：7365-45-9

Chinese name：HEPES

Size	Price
Free Sample (0.1 - 0.2 mg)		Inquiry
5 g		Inquiry
10 g		Inquiry
500 mg	￥500.00	AddToCart
1 g	￥700.00	AddToCart

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Biological activity
Information

Biological activity

Bioactivity

HEPES, a nonvolatile zwitterionic chemical buffering agent, is broadly applied in cell culture. HEPES is effective at pH 6.8 to 8.2. HEPES is also a potent inducer of lysosome biogenesis.

CAS

7365-45-9

Chinese name

HEPES

Mol.Wt.

238.30

In vitro

HEPES maintains superhydrophilicity of titanium for at least 3 months and resulted in a continuous retention of bioactivity and osteoconductivity.
HEPES drives lysosome biogenesis, affects MiT/TFE cytoplasmic-nuclear distribution, disrupts global cellular transcriptional profiles, resulting the activation of a MiT/TFE-dependent lysosomal-autophagic gene network in cultured RAW264.7 cells.

The accuracy of these methods have not been independently confirmed. They are for reference only.

Format

Solid

Transportation

Room temperature in continental US; may vary elsewhere.

Solubility

In Vitro:

H₂O : 250 mg/mL (1049.10 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	4.1964 mL	20.9820 mL	41.9639 mL
5 mM	0.8393 mL	4.1964 mL	8.3928 mL
10 mM	0.4196 mL	2.0982 mL	4.1964 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.
请依序添加每种溶剂： PBS
Solubility: 100 mg/mL (419.64 mM); Clear solution; Need ultrasonic

*以上所有助溶剂都可在 MCE 网站选购。

Purity

≥98.0%

Information

Intro	HEPES 是一种非挥发的两性离子化学缓冲剂，广泛应用于细胞培养。HEPES 在 pH 值为 6.8 至 8.2 时为有效的缓冲液。HEPES 也是溶酶体生物发生的有效诱导剂。
Synonym	HEPES
Rela_targets	Bioactive Compound Library Plus

AYB26681