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HAND1 Antibody  (Cat.#:AYP5000)

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Host: RabbitClonality: PolyclonalReactivity: Human,Mouse,RatIFELISA
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货号 AYP5000
靶点/基因 HAND1
宿主 Rabbit
克隆性 Polyclonal
反应种属 Human, Mouse, Rat
应用 IF, ELISA

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Cat.#：AYP5000

SizePrice
50 μL ￥1150.00 AddToCart
100 μL ￥2100.00 AddToCart

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  • Information

  • 应用指南

  • Related products

  • Immunogen

  • Target data

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  • FAQs

Reactivity Human,Mouse,Rat
Host Rabbit
Clonality Polyclonal
同种型 IgG
Application IFELISA
Dilution IF: 1:50 - 1:200
Predicted MW 23kDa
Observed MW
Format Liquid
Storage Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.75% BSA,50% glycerol,pH7.3.
Conjugate Unconjugated
Positive SH-SY5Y,A-549,NIH/3T3
Subcellular Nucleus,nucleolus,nucleoplasm
Purification Affinity purification

应用与推荐条件

快速判断怎么用

以下条件基于推荐浓度、验证图说明与通用实验要求整理，可作为预实验起点；不同样本和检测体系建议做梯度优化。

IF IF 推荐条件
推荐稀释 1:50 - 1:200
建议样本/阳性对照 SH-SY5Y,A-549,NIH/3T3
关键条件 建议优化固定、通透和封闭条件，并设置二抗空白对照
预期结果 预期荧光定位：Nucleus,nucleolus,nucleoplasm
对照设置 建议设置阳性样本、阴性样本和二抗/同型对照
ELISA ELISA 推荐条件
推荐稀释 请参考验证图说明或咨询技术支持
建议样本/阳性对照 SH-SY5Y,A-549,NIH/3T3
关键条件 建议从页面推荐浓度开始，结合样本与检测体系做梯度优化
预期结果 预期信号/条带约 23kDa
对照设置 建议设置阳性样本、阴性样本和二抗/同型对照

Related products

More associated products >>
HAND1 Rabbit pAb
同靶点
AYP13180
HAND1 Rabbit pAb
WB

Immunogen

Immunogen Synthesized peptide derived from Human HAND1.
Sequence
查看序列 
PHPAHPMLHEPFLFGPASRCHQERPYFQSWLLSPADAAPDFPAGGPPPAAAAAATAYGPDARPGQSPGRLEALGGRLGRRKGSGPKKERRRTESINSAFAELRECIPNVPADTKLSKIKTLRLATSYIAYLMDVLAKDAQSGDPEAFKAELKKADGGRESKRKRELQQHEGFPPALGPVEKRIKGRTGWPQQVWALELNQ

Target data

Background The protein encoded by this gene belongs to the basic helix-loop-helix family of transcription factors. This gene product is one of two closely related family members, the HAND proteins, which are asymmetrically expressed in the developing ventricular chambers and play an essential role in cardiac morphogenesis. Working in a complementary fashion, they function in the formation of the right ventricle and aortic arch arteries, implicating them as mediators of congenital heart disease. In addition, it has been suggested that this transcription factor may be required for early trophoblast differentiation.
Gene ID 9421
Gene name HAND1
Swiss O96004
Synonym HAND1,Hxt,Thing1,bHLHa27,eHand,HAND1 Antibody,Class A basic helix-loop-helix protein 27,Extraembryonic tissues,heart,autonomic nervous system and neural crest derivatives-expressed protein 1
Expression Heart.
Functions Transcription factor that plays an essential role in both trophoblast-giant cells differentiation and in cardiac morphogenesis. In the adult, could be required for ongoing expression of cardiac-specific genes. Binds the DNA sequence 5'-NRTCTG-3' (non-canonical E-box) (By similarity).
Research area

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FAQs

当前页面标注应用包括 IF, ELISA，建议结合页面验证图和推荐稀释比例进行预实验优化。
可通过页面询价/留言入口提交货号和批号，技术支持会协助提供对应批次资料。
页面推荐条件可作为起始浓度，不同样本、固定方式和检测体系可能需要梯度优化。
AYP5000