Fbxw7 (YD19005) Rabbit mAb (Cat.#:AYD12454)
A
小A
销售顾问
请使用微信扫一扫
添加顾问进行咨询
货号
AYD12454
靶点/基因
FBXW7
宿主
Rabbit
克隆性
Monoclonal
反应种属
Human, Mouse, Rat
应用
IHC-P, FC
暂无验证图
欢迎提交实验图片，或联系 Support 获取更多信息。
Information
应用指南
Related products
Immunogen
Target data
资料与支持
FAQs
| Reactivity | Human,Mouse,Rat |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| 同种型 | IgG |
| Application | IHC-PFC |
| Dilution | |
| Predicted MW | 80kDa |
| Observed MW | |
| Format | Liquid |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.75% BSA,50% glycerol,pH7.3. |
| Conjugate | Unconjugated |
| Positive | HepG2,293F,Mouse brain,Rat brain |
| Subcellular | Nucleus, nucleoplasm, Chromosome, Cytoplasm, nucleolus |
| Purification | 亲和纯化 |
应用与推荐条件
快速判断怎么用
以下条件基于推荐浓度、验证图说明与通用实验要求整理，可作为预实验起点；不同样本和检测体系建议做梯度优化。
IHC-P
IHC-P 推荐条件
| 推荐稀释 | 请参考验证图说明或咨询技术支持 |
|---|---|
| 建议样本/阳性对照 | HepG2,293F,Mouse brain,Rat brain |
| 关键条件 | 石蜡切片建议优化抗原修复液 pH、修复时间和一抗孵育条件 |
| 预期结果 | 预期定位：Nucleus, nucleoplasm, Chromosome, Cytoplasm, nucleolus |
| 对照设置 | 建议设置阳性样本、阴性样本和二抗/同型对照 |
FC
FC 推荐条件
| 推荐稀释 | 请参考验证图说明或咨询技术支持 |
|---|---|
| 建议样本/阳性对照 | HepG2,293F,Mouse brain,Rat brain |
| 关键条件 | 如检测胞内靶点，需优化固定/通透条件，并设置同型对照 |
| 预期结果 | 预期阳性群体荧光信号相对阴性/同型对照右移 |
| 对照设置 | 建议设置阳性样本、阴性样本和二抗/同型对照 |
Related products
More associated products >>
Immunogen
| Immunogen | Please contact Support |
|---|---|
| Sequence | Email For Sequence |
Target data
| Background | This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene was previously referred to as FBX30, and belongs to the Fbws class; in addition to an F-box, this protein contains 7 tandem WD40 repeats. This protein binds directly to cyclin E and probably targets cyclin E for ubiquitin-mediated degradation. Mutations in this gene are detected in ovarian and breast cancer cell lines, implicating the gene's potential role in the pathogenesis of human cancers. Multiple transcript variants encoding different isoforms have been found for this gene. |
|---|---|
| Gene ID | 55294 |
| Gene name | FBXW7 |
| Swiss | Q969H0 |
| Synonym | Fbxw7 (YD19005),Fbxw7 (YD19005) Rabbit mAb,FBXW7,Archipelago homolog,F-box and WD-40 domain-containing protein 7,F-box protein FBX30,SEL-10,hCdc4,FBW7 |
资料与技术支持
验证数据
欢迎联系技术支持获取验证图与应用案例。
FAQs
当前页面标注应用包括 IHC-P, FC，建议结合页面验证图和推荐稀释比例进行预实验优化。
可通过页面询价/留言入口提交货号和批号，技术支持会协助提供对应批次资料。
页面推荐条件可作为起始浓度，不同样本、固定方式和检测体系可能需要梯度优化。