CD81 (YD15729) Rabbit mAb (Cat.#:AYD11810)
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Cat.#
AYD11810
Target / gene
CD81
Host
Rabbit
Clonality
Monoclonal
Reactivity
Human
Application
IHC-P, ICC/IF, FC
Cat.#：AYD11810
| Size | Price | |
|---|---|---|
| 50ul | RMB: 680Sale1280 | Add to cart |
| 100ul | RMB: 1219Sale2300 | Add to cart |
Sales Terms | Request COA | Stock and shipping are confirmed by support
Information
Application guide
Related products
Immunogen
Target data
Resources
FAQs
| Reactivity | Human |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Application | IHC-PICC/IFFC |
| Dilution | |
| Predicted MW | 26kDa |
| Observed MW | |
| Format | Liquid |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.75% BSA,50% glycerol,pH7.3. |
| Conjugate | Unconjugated |
| Positive | Rat brain |
| Subcellular | Cell membrane, Basolateral cell membrane |
| Purification | 亲和纯化 |
Applications and conditions
Where to start
These starting conditions come from the recommended dilution, validation images, and general assay practice. Titrate for your sample and detection system.
IHC-P
Conditions for IHC-P
| Recommended dilution | See the validation images or contact technical support |
|---|---|
| Suggested sample / positive control | Rat brain |
| Key conditions | For paraffin sections, optimize retrieval buffer pH, retrieval time, and primary incubation |
| Expected result | Expected localization: Cell membrane, Basolateral cell membrane |
| Controls | Include a positive sample, a negative sample, and a secondary or isotype control |
ICC/IF
Conditions for ICC/IF
| Recommended dilution | See the validation images or contact technical support |
|---|---|
| Suggested sample / positive control | Rat brain |
| Key conditions | Optimize fixation, permeabilization, and blocking, and include a secondary-only control |
| Expected result | Expected fluorescence localization: Cell membrane, Basolateral cell membrane |
| Controls | Include a positive sample, a negative sample, and a secondary or isotype control |
FC
Conditions for FC
| Recommended dilution | See the validation images or contact technical support |
|---|---|
| Suggested sample / positive control | Rat brain |
| Key conditions | For intracellular targets, optimize fixation and permeabilization, and include an isotype control |
| Expected result | Expect the positive population to shift relative to the negative or isotype control |
| Controls | Include a positive sample, a negative sample, and a secondary or isotype control |
Related products
More associated products >>
Immunogen
| Immunogen | Please contact Support |
|---|---|
| Sequence | Request sequence |
Target data
| Background | The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. This protein appears to promote muscle cell fusion and support myotube maintenance. Also it may be involved in signal transduction. This gene is localized in the tumor-suppressor gene region and thus it is a candidate gene for malignancies. Two transcript variants encoding different isoforms have been found for this gene. |
|---|---|
| Gene ID | 975 |
| Gene name | CD81 |
| Swiss | P60033 |
| Synonym | CD81 (YD15729),CD81 (YD15729) Rabbit mAb,CD81,26 kDa cell surface protein TAPA-1,Target of the antiproliferative antibody 1,Tetraspanin-28,TAPA1,TSPAN28 |
Resources
Validation data
1 images
Validation images at the top of the page cover IHC-P / ICC/IF / FC.
References
No papers are linked yet. Contact technical support for application examples.
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Support center
Need help with the application?
Send the catalog number, application, sample type, and assay. Support can confirm dilution, controls, and COA or lot documents.
FAQs
Listed applications: IHC-P, ICC/IF, FC. Use the validation images and recommended dilution as a starting point.
Send the catalog number and lot through the inquiry form. Support will help with the lot documents.
Use the recommended conditions as a starting concentration. Sample, fixation, and detection system may need a titration.