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CD47 (YD20943) Rabbit mAb  (Cat.#:AYD12754)

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Host: RabbitClonality: MonoclonalReactivity: HumanICC/IFFC
Cat.# AYD12754
Target / gene CD47
Host Rabbit
Clonality Monoclonal
Reactivity Human
Application ICC/IF, FC

Cat.#:AYD12754

SizePrice
50ul RMB: 680Sale1280 Add to cart
100ul RMB: 1219Sale2300 Add to cart
  • Information

  • Application guide

  • Related products

  • Immunogen

  • Target data

  • Resources

  • FAQs

Reactivity Human
Host Rabbit
Clonality Monoclonal
Isotype IgG
Application ICC/IFFC
Dilution
Predicted MW 35kDa
Observed MW
Format Liquid
Storage Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.75% BSA,50% glycerol,pH7.3.
Conjugate Unconjugated
Positive U-251MG,Mouse brain,Rat brain
Subcellular Cell membrane
Purification 亲和纯化

Applications and conditions

Where to start

These starting conditions come from the recommended dilution, validation images, and general assay practice. Titrate for your sample and detection system.

ICC/IF Conditions for ICC/IF
Recommended dilution See the validation images or contact technical support
Suggested sample / positive control U-251MG,Mouse brain,Rat brain
Key conditions Optimize fixation, permeabilization, and blocking, and include a secondary-only control
Expected result Expected fluorescence localization: Cell membrane
Controls Include a positive sample, a negative sample, and a secondary or isotype control
FC Conditions for FC
Recommended dilution See the validation images or contact technical support
Suggested sample / positive control U-251MG,Mouse brain,Rat brain
Key conditions For intracellular targets, optimize fixation and permeabilization, and include an isotype control
Expected result Expect the positive population to shift relative to the negative or isotype control
Controls Include a positive sample, a negative sample, and a secondary or isotype control

Related products

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Immunogen

Immunogen Please contact Support
Sequence Request sequence

Target data

Background This gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes. Alternatively spliced transcript variants have been found for this gene.
Gene ID 961
Gene name CD47
Swiss Q08722
Synonym CD47 (YD20943),CD47 (YD20943) Rabbit mAb,CD47,Antigenic surface determinant protein OA3,Integrin-associated protein,Protein MER6,MER6

Resources

Validation data

Contact technical support for validation images and application examples.

References

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Support

Support can help confirm the application, sample, dilution, controls, and assay conditions.

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Need help with the application?

Send the catalog number, application, sample type, and assay. Support can confirm dilution, controls, and COA or lot documents.

FAQs

Listed applications: ICC/IF, FC. Use the validation images and recommended dilution as a starting point.
Send the catalog number and lot through the inquiry form. Support will help with the lot documents.
Use the recommended conditions as a starting concentration. Sample, fixation, and detection system may need a titration.
AYD12754