CD27 (YD14413) Rabbit mAb (Cat.#:AYD15310)
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货号
AYD15310
靶点/基因
Cd27
宿主
Rabbit
克隆性
Monoclonal
反应种属
Mouse
应用
IHC-P
暂无验证图
欢迎提交实验图片，或联系 Support 获取更多信息。
Information
应用指南
Immunogen
Target data
资料与支持
FAQs
| Reactivity | Mouse |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| 同种型 | IgG |
| Application | IHC-P |
| Dilution | |
| Predicted MW | 28kDa |
| Observed MW | |
| Format | Liquid |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.75% BSA,50% glycerol,pH7.3. |
| Conjugate | Unconjugated |
| Positive | |
| Subcellular | Cell membrane |
| Purification | 亲和纯化 |
应用与推荐条件
快速判断怎么用
以下条件基于推荐浓度、验证图说明与通用实验要求整理，可作为预实验起点；不同样本和检测体系建议做梯度优化。
IHC-P
IHC-P 推荐条件
| 推荐稀释 | 请参考验证图说明或咨询技术支持 |
|---|---|
| 建议样本/阳性对照 | 建议选择靶点高表达样本作为阳性对照 |
| 关键条件 | 石蜡切片建议优化抗原修复液 pH、修复时间和一抗孵育条件 |
| 预期结果 | 预期定位：Cell membrane |
| 对照设置 | 建议设置阳性样本、阴性样本和二抗/同型对照 |
Immunogen
| Immunogen | Please contact Support |
|---|---|
| Sequence | Email For Sequence |
Target data
| Background | Costimulatory immune-checkpoint receptor expressed at the surface of T-cells, NK-cells and B-cells which binds to and is activated by its ligand CD70/CD27L expressed by B-cells. The CD70-CD27 signaling pathway mediates antigen-specific T-cell activation and expansion which in turn provides immune surveillance of B-cells (PubMed:11062504). Mechanistically, CD70 ligation activates the TRAF2-PTPN6 axis that subsequently inhibits LCK phosphorylation to promote phenotypic and transcriptional adaptations of T-cell memory (By similarity). In addition, activation by CD70 on early progenitor cells provides a negative feedback signal to leukocyte differentiation during immune activation and thus modulates hematopoiesis (PubMed:15723067). Negatively regulates the function of Th2 lymphocytes in the adipose tissue (PubMed:38386557) |
|---|---|
| Gene ID | 3134 |
| Gene name | Cd27 |
| Swiss | P41272 |
| Synonym | CD27 (YD14413),CD27 (YD14413) Rabbit mAb,Cd27,CD27L receptor,T-cell activation antigen CD27,Tumor necrosis factor receptor superfamily member 7,Tnfrsf7 |
资料与技术支持
验证数据
欢迎联系技术支持获取验证图与应用案例。
FAQs
当前页面标注应用包括 IHC-P，建议结合页面验证图和推荐稀释比例进行预实验优化。
可通过页面询价/留言入口提交货号和批号，技术支持会协助提供对应批次资料。
页面推荐条件可作为起始浓度，不同样本、固定方式和检测体系可能需要梯度优化。