Aquaporin 3 (YD14114) Rabbit mAb (Cat.#:AYD11138)
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Immunofluorescence analysis of A431 cells labelling Aquaporin 3 with Aquaporin 3 The cells were fixed with cold 100% methanol (10min, 4℃) and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with Aquaporin 3 (1:100) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 . Nuclear DNA was labeled in blue with DAPI.
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Aquaporin 3 (YD14114) Rabbit mAb
Immunofluorescence analysis of A431 cells labelling Aquaporin 3 with Aquaporin 3 The cells were fixed with cold 100% methanol (10min, 4℃) and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with Aquaporin 3 (1:100) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 . Nuclear DNA was labeled in blue with DAPI.
Cat.#：AYD11138
| Size | Price | |
|---|---|---|
| 50ul | RMB: 680Sale1280 | Add to cart |
| 100ul | RMB: 1219Sale2300 | Add to cart |
Sales Terms | Request COA | Stock and shipping are confirmed by support
Information
Application guide
Related products
Immunogen
Target data
Resources
FAQs
| Reactivity | Human |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Application | IHC-PICC/IF |
| Dilution | IHC-P: 1:100-1:500: ICC/IF: 1:100-1:300: |
| Predicted MW | 32kDa |
| Observed MW | 32kDa |
| Format | Liquid |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.75% BSA,50% glycerol,pH7.3. |
| Conjugate | Unconjugated |
| Positive | Mouse brain,Rat brain |
| Subcellular | Cell membrane, Basolateral cell membrane |
| Purification | 亲和纯化 |
Applications and conditions
These starting conditions come from the recommended dilution, validation images, and general assay practice. Titrate for your sample and detection system.
| Recommended dilution | See the validation images or contact technical support |
|---|---|
| Suggested sample / positive control | Mouse brain,Rat brain |
| Key conditions | For paraffin sections, optimize retrieval buffer pH, retrieval time, and primary incubation |
| Expected result | Expected localization: Cell membrane, Basolateral cell membrane |
| Controls | Include a positive sample, a negative sample, and a secondary or isotype control |
| Recommended dilution | See the validation images or contact technical support |
|---|---|
| Suggested sample / positive control | Mouse brain,Rat brain |
| Key conditions | Optimize fixation, permeabilization, and blocking, and include a secondary-only control |
| Expected result | Expected fluorescence localization: Cell membrane, Basolateral cell membrane |
| Controls | Include a positive sample, a negative sample, and a secondary or isotype control |
Related products
More associated products >>
Immunogen
| Immunogen | Please contact Support |
|---|---|
| Sequence | Request sequence |
Target data
| Background | This gene encodes the water channel protein aquaporin 3. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein, also known as aquaporin 0. Aquaporin 3 is localized at the basal lateral membranes of collecting duct cells in the kidney. In addition to its water channel function, aquaporin 3 has been found to facilitate the transport of nonionic small solutes such as urea and glycerol, but to a smaller degree. It has been suggested that water channels can be functionally heterogeneous and possess water and solute permeation mechanisms. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. |
|---|---|
| Gene ID | 360 |
| Gene name | AQP3 |
| Swiss | Q92482 |
| Synonym | Aquaporin 3 (YD14114),Aquaporin 3 (YD14114) Rabbit mAb,AQP3,Aquaglyceroporin-3 |
Resources
Validation data
1 images
Validation images at the top of the page cover IHC-P / ICC/IF.
References
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