Adrenergic Receptor α-2B Antibody (Cat.#:AYP5053)
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货号
AYP5053
靶点/基因
ADRA2B
宿主
Rabbit
克隆性
Polyclonal
反应种属
Human
应用
IF, ELISA
暂无验证图
欢迎提交实验图片，或联系 Support 获取更多信息。
Information
应用指南
Related products
Immunogen
Target data
资料与支持
FAQs
| Reactivity | Human |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| 同种型 | IgG |
| Application | IFELISA |
| Dilution | IF: 1:50 - 1:200 |
| Predicted MW | 49kDa |
| Observed MW | |
| Format | Liquid |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.75% BSA,50% glycerol,pH7.3. |
| Conjugate | Unconjugated |
| Positive | U-87MG,Mouse skeletal muscle,Mouse kidney,Rat liver,Rat kidney |
| Subcellular | Cell membrane,Multi-pass membrane protein |
| Purification | Affinity purification |
应用与推荐条件
快速判断怎么用
以下条件基于推荐浓度、验证图说明与通用实验要求整理，可作为预实验起点；不同样本和检测体系建议做梯度优化。
IF
IF 推荐条件
| 推荐稀释 | 1:50 - 1:200 |
|---|---|
| 建议样本/阳性对照 | U-87MG,Mouse skeletal muscle,Mouse kidney,Rat liver,Rat kidney |
| 关键条件 | 建议优化固定、通透和封闭条件，并设置二抗空白对照 |
| 预期结果 | 预期荧光定位：Cell membrane,Multi-pass membrane protein |
| 对照设置 | 建议设置阳性样本、阴性样本和二抗/同型对照 |
ELISA
ELISA 推荐条件
| 推荐稀释 | 请参考验证图说明或咨询技术支持 |
|---|---|
| 建议样本/阳性对照 | U-87MG,Mouse skeletal muscle,Mouse kidney,Rat liver,Rat kidney |
| 关键条件 | 建议从页面推荐浓度开始，结合样本与检测体系做梯度优化 |
| 预期结果 | 预期信号/条带约 49kDa |
| 对照设置 | 建议设置阳性样本、阴性样本和二抗/同型对照 |
Related products
More associated products >>
Immunogen
| Immunogen | Synthesized peptide derived from Human Adrenergic Receptor α-2B. |
|---|---|
| Sequence |
查看序列MDHQDPYSVQATAAIAAAITFLILFTIFGNALVILAVLTSRSLRAPQNLFLVSLAAADILVATLIIPFSLANELLGYWYFRRTWCEVYLALDVLFCTSSI |
Target data
| Background | This intronless gene encodes a seven-pass transmembrane protein. This protein is a member of a subfamily of G protein-coupled receptors that regulate neurotransmitter release from sympathetic nerves and from adrenergic neurons in the central nervous system. |
|---|---|
| Gene ID | 151 |
| Gene name | ADRA2B |
| Swiss | P18089 |
| Synonym | ADRA2B,ADRA2L1,ADRA2RL1,ADRARL1,ALPHA2BAR,FAME2,alpha-2BAR,Adrenergic Receptor α-2B Antibody,Alpha-2 adrenergic receptor subtype C2,Alpha-2B adrenoreceptor,Adrenergic Receptor α-2B |
| Functions | Alpha-2 adrenergic receptors mediate the catecholamine-induced inhibition of adenylate cyclase through the action of G proteins. The rank order of potency for agonists of this receptor is clonidine > norepinephrine > epinephrine = oxymetazoline > dopamine > p-tyramine = phenylephrine > serotonin > p-synephrine / p-octopamine. For antagonists, the rank order is yohimbine > chlorpromazine > phentolamine > mianserine > spiperone > prazosin > alprenolol > propanolol > pindolol. |
| Research area |
资料与技术支持
验证数据
欢迎联系技术支持获取验证图与应用案例。
FAQs
当前页面标注应用包括 IF, ELISA，建议结合页面验证图和推荐稀释比例进行预实验优化。
可通过页面询价/留言入口提交货号和批号，技术支持会协助提供对应批次资料。
页面推荐条件可作为起始浓度，不同样本、固定方式和检测体系可能需要梯度优化。