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AKAP3 Antibody  (Cat.#:AYP4985)

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Host: RabbitClonality: PolyclonalReactivity: Human,MouseIFELISA
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Cat.# AYP4985
Target / gene AKAP3
Host Rabbit
Clonality Polyclonal
Reactivity Human, Mouse
Application IF, ELISA

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Cat.#：AYP4985

SizePrice
50 μL ￥1150.00 Add to cart
100 μL ￥2100.00 Add to cart

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  • Information

  • Application guide

  • Related products

  • Immunogen

  • Target data

  • Resources

  • FAQs

Reactivity Human,Mouse
Host Rabbit
Clonality Polyclonal
Isotype IgG
Application IFELISA
Dilution IF: 1:50 - 1:200
Predicted MW 94kDa
Observed MW
Format Liquid
Storage Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.75% BSA,50% glycerol,pH7.3.
Conjugate Unconjugated
Positive Rat testis
Subcellular Cytoplasmic vesicle,acrosome,secretory vesicle
Purification Affinity purification

Applications and conditions

Where to start

These starting conditions come from the recommended dilution, validation images, and general assay practice. Titrate for your sample and detection system.

IF Conditions for IF
Recommended dilution 1:50 - 1:200
Suggested sample / positive control Rat testis
Key conditions Optimize fixation, permeabilization, and blocking, and include a secondary-only control
Expected result Expected fluorescence localization: Cytoplasmic vesicle,acrosome,secretory vesicle
Controls Include a positive sample, a negative sample, and a secondary or isotype control
ELISA Conditions for ELISA
Recommended dilution See the validation images or contact technical support
Suggested sample / positive control Rat testis
Key conditions Start at the recommended dilution and titrate for your sample and assay
Expected result Expected signal or band near 94kDa
Controls Include a positive sample, a negative sample, and a secondary or isotype control

Related products

More associated products >>
AKAP3 Rabbit pAb
Same target
AYP17664
AKAP3 Rabbit pAb
WB

Immunogen

Immunogen Synthesized peptide derived from Human AKAP3.
Sequence
View sequence 
MSEKVDWLQSQNGVCKVDVYSPGDNQAQDWKMDTSTDPVRVLSWLRRDLEKSTAEFQDVRFKPGESFGGETSNSGDPHKGFSVDYYNTTTKGTPERLHFEMTHKEIPCQGPRAQLGNGSSVDEVSFYANRLTNLVIAMARKEINEKIDGSENKCVYQSLYMGNEPTPTKSLSKIASELVNETVSACSRNAAPDKAPGSGDRVSGSSQSPPNLKYKSTLKIKESTKERQGPDDKPPSKKSFFYKEVFESRNGDYAREGGRFFPRERKRFRGQERPDDFTASVSEGIMTYANSVVSDMMVSI

Target data

Background This gene encodes a member of A-kinase anchoring proteins (AKAPs), a family of functionally related proteins that target protein kinase A to discrete locations within the cell. The encoded protein is reported to participate in protein-protein interactions with the R-subunit of the protein kinase A as well as sperm-associated proteins. This protein is expressed in spermatozoa and localized to the acrosomal region of the sperm head as well as the length of the principal piece. It may function as a regulator of motility, capacitation, and the acrosome reaction.
Gene ID 10566
Gene name AKAP3
Swiss O75969
Synonym AKAP3,AKAP 110,AKAP110,CT82,FSP95,HEL159,PRKA3,SOB1,AKAP3 Antibody,A-kinase anchor protein 110 kDa,Cancer/testis antigen 82,Fibrous sheath protein of 95 kDa,Fibrousheathin I,Fibrousheathin-1,Protein kinase A-anchoring protein 3,Sperm oocyte-binding protein
Expression Testis specific; only expressed in spermatids.
Functions May function as a regulator of both motility- and head-associated functions such as capacitation and the acrosome reaction.

Resources

Validation data

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References

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FAQs

Listed applications: IF, ELISA. Use the validation images and recommended dilution as a starting point.
Send the catalog number and lot through the inquiry form. Support will help with the lot documents.
Use the recommended conditions as a starting concentration. Sample, fixation, and detection system may need a titration.
AYP4985