AKAP3 Antibody (Cat.#:AYP4985)
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货号
AYP4985
靶点/基因
AKAP3
宿主
Rabbit
克隆性
Polyclonal
反应种属
Human, Mouse
应用
IF, ELISA
暂无验证图
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Information
应用指南
Related products
Immunogen
Target data
资料与支持
FAQs
| Reactivity | Human,Mouse |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| 同种型 | IgG |
| Application | IFELISA |
| Dilution | IF: 1:50 - 1:200 |
| Predicted MW | 94kDa |
| Observed MW | |
| Format | Liquid |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.75% BSA,50% glycerol,pH7.3. |
| Conjugate | Unconjugated |
| Positive | Rat testis |
| Subcellular | Cytoplasmic vesicle,acrosome,secretory vesicle |
| Purification | Affinity purification |
应用与推荐条件
快速判断怎么用
以下条件基于推荐浓度、验证图说明与通用实验要求整理，可作为预实验起点；不同样本和检测体系建议做梯度优化。
IF
IF 推荐条件
| 推荐稀释 | 1:50 - 1:200 |
|---|---|
| 建议样本/阳性对照 | Rat testis |
| 关键条件 | 建议优化固定、通透和封闭条件，并设置二抗空白对照 |
| 预期结果 | 预期荧光定位：Cytoplasmic vesicle,acrosome,secretory vesicle |
| 对照设置 | 建议设置阳性样本、阴性样本和二抗/同型对照 |
ELISA
ELISA 推荐条件
| 推荐稀释 | 请参考验证图说明或咨询技术支持 |
|---|---|
| 建议样本/阳性对照 | Rat testis |
| 关键条件 | 建议从页面推荐浓度开始，结合样本与检测体系做梯度优化 |
| 预期结果 | 预期信号/条带约 94kDa |
| 对照设置 | 建议设置阳性样本、阴性样本和二抗/同型对照 |
Related products
More associated products >>
Immunogen
| Immunogen | Synthesized peptide derived from Human AKAP3. |
|---|---|
| Sequence |
查看序列MSEKVDWLQSQNGVCKVDVYSPGDNQAQDWKMDTSTDPVRVLSWLRRDLEKSTAEFQDVRFKPGESFGGETSNSGDPHKGFSVDYYNTTTKGTPERLHFEMTHKEIPCQGPRAQLGNGSSVDEVSFYANRLTNLVIAMARKEINEKIDGSENKCVYQSLYMGNEPTPTKSLSKIASELVNETVSACSRNAAPDKAPGSGDRVSGSSQSPPNLKYKSTLKIKESTKERQGPDDKPPSKKSFFYKEVFESRNGDYAREGGRFFPRERKRFRGQERPDDFTASVSEGIMTYANSVVSDMMVSI |
Target data
| Background | This gene encodes a member of A-kinase anchoring proteins (AKAPs), a family of functionally related proteins that target protein kinase A to discrete locations within the cell. The encoded protein is reported to participate in protein-protein interactions with the R-subunit of the protein kinase A as well as sperm-associated proteins. This protein is expressed in spermatozoa and localized to the acrosomal region of the sperm head as well as the length of the principal piece. It may function as a regulator of motility, capacitation, and the acrosome reaction. |
|---|---|
| Gene ID | 10566 |
| Gene name | AKAP3 |
| Swiss | O75969 |
| Synonym | AKAP3,AKAP 110,AKAP110,CT82,FSP95,HEL159,PRKA3,SOB1,AKAP3 Antibody,A-kinase anchor protein 110 kDa,Cancer/testis antigen 82,Fibrous sheath protein of 95 kDa,Fibrousheathin I,Fibrousheathin-1,Protein kinase A-anchoring protein 3,Sperm oocyte-binding protein |
| Expression | Testis specific; only expressed in spermatids. |
| Functions | May function as a regulator of both motility- and head-associated functions such as capacitation and the acrosome reaction. |
资料与技术支持
验证数据
欢迎联系技术支持获取验证图与应用案例。
FAQs
当前页面标注应用包括 IF, ELISA，建议结合页面验证图和推荐稀释比例进行预实验优化。
可通过页面询价/留言入口提交货号和批号，技术支持会协助提供对应批次资料。
页面推荐条件可作为起始浓度，不同样本、固定方式和检测体系可能需要梯度优化。