ABCB11/BSEP (YD20810) Rabbit mAb (Cat.#:AYD12767)
A
货号
AYD12767
靶点/基因
ABCB11
宿主
Rabbit
克隆性
Monoclonal
反应种属
Human
应用
IHC-P, ICC/IF, FC
暂无验证图
欢迎提交实验图片,或联系 Support 获取更多信息。
Information
应用指南
Related products
Immunogen
Target data
资料与支持
FAQs
| Reactivity | Human |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| 同种型 | IgG |
| Application | IHC-PICC/IFFC |
| Dilution | |
| Predicted MW | 146kDa |
| Observed MW | |
| Format | Liquid |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.75% BSA,50% glycerol,pH7.3. |
| Conjugate | Unconjugated |
| Positive | Rat liver |
| Subcellular | Apical cell membrane, Recycling endosome membrane, Endosome, Cell membrane |
| Purification | 亲和纯化 |
应用与推荐条件
快速判断怎么用
以下条件基于推荐浓度、验证图说明与通用实验要求整理,可作为预实验起点;不同样本和检测体系建议做梯度优化。
IHC-P
IHC-P 推荐条件
| 推荐稀释 | 请参考验证图说明或咨询技术支持 |
|---|---|
| 建议样本/阳性对照 | Rat liver |
| 关键条件 | 石蜡切片建议优化抗原修复液 pH、修复时间和一抗孵育条件 |
| 预期结果 | 预期定位:Apical cell membrane, Recycling endosome membrane, Endosome, Cell membrane |
| 对照设置 | 建议设置阳性样本、阴性样本和二抗/同型对照 |
ICC/IF
ICC/IF 推荐条件
| 推荐稀释 | 请参考验证图说明或咨询技术支持 |
|---|---|
| 建议样本/阳性对照 | Rat liver |
| 关键条件 | 建议优化固定、通透和封闭条件,并设置二抗空白对照 |
| 预期结果 | 预期荧光定位:Apical cell membrane, Recycling endosome membrane, Endosome, Cell membrane |
| 对照设置 | 建议设置阳性样本、阴性样本和二抗/同型对照 |
FC
FC 推荐条件
| 推荐稀释 | 请参考验证图说明或咨询技术支持 |
|---|---|
| 建议样本/阳性对照 | Rat liver |
| 关键条件 | 如检测胞内靶点,需优化固定/通透条件,并设置同型对照 |
| 预期结果 | 预期阳性群体荧光信号相对阴性/同型对照右移 |
| 对照设置 | 建议设置阳性样本、阴性样本和二抗/同型对照 |
Related products
More associated products >>Immunogen
| Immunogen | Please contact Support |
|---|---|
| Sequence | Email For Sequence |
Target data
| Background | The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is the major canalicular bile salt export pump in man. Mutations in this gene cause a form of progressive familial intrahepatic cholestases which are a group of inherited disorders with severe cholestatic liver disease from early infancy. |
|---|---|
| Gene ID | 8647 |
| Gene name | ABCB11 |
| Swiss | O95342 |
| Synonym | ABCB11/BSEP (YD20810),ABCB11/BSEP (YD20810) Rabbit mAb,ABCB11,ATP-binding cassette sub-family B member 11,BSEP |
资料与技术支持
验证数据
欢迎联系技术支持获取验证图与应用案例。
FAQs
当前页面标注应用包括 IHC-P, ICC/IF, FC,建议结合页面验证图和推荐稀释比例进行预实验优化。
可通过页面询价/留言入口提交货号和批号,技术支持会协助提供对应批次资料。
页面推荐条件可作为起始浓度,不同样本、固定方式和检测体系可能需要梯度优化。