4-Nitrophenyl α-D-galactopyranoside (Cat.#:AYB26921)

Name: 4-Nitrophenyl α-D-galactopyranoside
Brand: 艾博立生物
SKU: AYB26921
Availability: InStock

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Cat.#：AYB26921

CAS：7493-95-0

Chinese name：4-硝基苯基 α-D-吡喃半乳糖苷

Size	Price
Free Sample (0.1 - 0.2 mg)		Inquiry
500 mg		Inquiry
1 g		Inquiry
100 mg	￥600.00	AddToCart
250 mg	￥1200.00	AddToCart

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Sales Terms

Biological activity
Information

Biological activity

Bioactivity

4-Nitrophenyl α-D-galactopyranoside (PNP-alpha-D-Gal) is an artificial substrate of 4-nitrophenyl (pNP) glycopyranoside for detecting α-galactosidase activity. The amount of released pNP is significantly increased when 4-Nitrophenyl α-D-galactopyranoside is used as substrates.

CAS

7493-95-0

Chinese name

4-硝基苯基 α-D-吡喃半乳糖苷

Mol.Wt.

301.25

In vitro

Using 4-nitorophenol (pNP)-glycopyranosides as pseudosubstrates, the amount of liberated pNP from the 4-Nitrophenyl α-D-galactopyranoside is significantly higher than that from the other pNP-glycopyranosides at pH 4.0 whereas no activity was detected at pH 7.0.
The digestion assays using 4-nitrophenyl (pNP) glycopyranosides as artificial substrates shows that yieldin liberated galactose residues from 4-Nitrophenyl α-D-galactopyranoside mainly at pH 4.0, i.e. the pH level where yield threshold is decreased at most.

The accuracy of these methods have not been independently confirmed. They are for reference only.

Format

Solid

Transportation

Room temperature in continental US; may vary elsewhere.

Solubility

In Vitro:

H₂O : 16.67 mg/mL (55.34 mM; ultrasonic and warming and heat to 60°C)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	3.3195 mL	16.5975 mL	33.1950 mL
5 mM	0.6639 mL	3.3195 mL	6.6390 mL
10 mM	0.3320 mL	1.6598 mL	3.3195 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.
请依序添加每种溶剂： PBS
Solubility: 25 mg/mL (82.99 mM); Clear solution; Need ultrasonic

*以上所有助溶剂都可在 MCE 网站选购。

Purity

98.53%

Information

Intro	4-Nitrophenyl α-D-galactopyranoside (PNP-alpha-D-Gal) 是一种 4-硝基苯基 (pNP) 糖吡喃糖苷的人工底物，用于检测 α-半乳糖苷酶活性。当 4-Nitrophenyl α-D-galactopyranoside 用作底物时，释放的 pNP 量会显着增加。
Synonym	PNP-alpha-D-Gal,PNP-α-D-Gal,4-硝基苯基 α-D-吡喃半乳糖苷
Rela_targets	Bioactive Compound Library Plus Glycoside Compound Library Heterocyclic Compound Library

AYB26921