4-Nitrophenyl phosphate disodium hexahydrate (Cat.#:AYB26831)
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Biological activity
Information
Biological activity
| Bioactivity | 4-Nitrophenyl phosphate (p-nitrophenyl phosphate) disodium hexahydrate is widely used as a small molecule phosphotyrosine-like substrate in activity assays for protein tyrosine phosphatases. 4-Nitrophenyl phosphate disodium hexahydrate is a colorless substrate that upon hydrolysis is converted to a yellow 4-nitrophenolate ion that can be monitored by absorbance at 405 nm. | ||||||||||||||||
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| CAS | 333338-18-4 | ||||||||||||||||
| Mol.Wt. | 371.14 | ||||||||||||||||
| In vitro | 4-Nitrophenyl phosphate (PNPP) disodium hexahydrate is a commonly used substrate for alkaline phosphatases (ALPs). 4-Nitrophenyl phosphate disodium hexahydrate is hydrolyzed by ALP to PNP (p-nitrophenol), which quenches the fluorescence of novel gold nanoclusters (AuNCs) by the inner filter effect (IFE). The accuracy of these methods have not been independently confirmed. They are for reference only. |
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| Format | Solid | ||||||||||||||||
| Transportation | Room temperature in continental US; may vary elsewhere. | ||||||||||||||||
| Storage | -20°C, sealed storage, away from moisture | ||||||||||||||||
| Solubility | In Vitro: H2O : 100 mg/mL (269.44 mM; Need ultrasonic) DMSO : 10 mg/mL (26.94 mM; ultrasonic and warming and heat to 80°C) 配制储备液
* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 |
Information
| Intro | 4-Nitrophenyl phosphate (p-nitrophenyl phosphate) disodium hexahydrate 广泛用作蛋白质酪氨酸磷酸酶活性测定中的小分子磷酸酪氨酸样底物。4-Nitrophenyl phosphate disodium hexahydrate 是一种无色基质，水解后转化为黄色的4-硝基苯酚离子，可在 405 nm 处通过吸光度监测。 |
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| Synonym | p-Nitrophenyl phosphate disodium hexahydrate |
| Rela_targets | Bioactive Compound Library Plus Oxygen Sensing Compound Library Human Metabolite Library |