VEGF Receptor 2 (YD20041) Rabbit mAb (货号:AYD12825)
A
货号
AYD12825
靶点/基因
Kdr
宿主
Rabbit
克隆性
Monoclonal
反应种属
Mouse
应用
ICC/IF, FC
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产品信息
应用指南
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抗原信息
靶点信息
资料与支持
常见问题
| 反应 | Mouse |
|---|---|
| 宿主 | Rabbit |
| 克隆性 | Monoclonal |
| 同种型 | IgG |
| 应用 | ICC/IFFC |
| 推荐浓度 | |
| 理论分子量 | 150kDa |
| 实测分子量 | |
| 形式 | Liquid |
| 保存条件 | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.75% BSA,50% glycerol,pH7.3. |
| 偶联物 | Unconjugated |
| 阳性对照 | |
| 细胞定位 | Cell junction, Endoplasmic reticulum, Cell membrane, Cytoplasm, Nucleus, Cytoplasmic vesicle, Early endosome, Secreted |
| 纯化 | 亲和纯化 |
应用与推荐条件
快速判断怎么用
以下条件基于推荐浓度、验证图说明与通用实验要求整理,可作为预实验起点;不同样本和检测体系建议做梯度优化。
ICC/IF
ICC/IF 推荐条件
| 推荐稀释 | 请参考验证图说明或咨询技术支持 |
|---|---|
| 建议样本/阳性对照 | 建议选择靶点高表达样本作为阳性对照 |
| 关键条件 | 建议优化固定、通透和封闭条件,并设置二抗空白对照 |
| 预期结果 | 预期荧光定位:Cell junction, Endoplasmic reticulum, Cell membrane, Cytoplasm, Nucleus, Cytoplasmic vesicle, Early endosome, Secreted |
| 对照设置 | 建议设置阳性样本、阴性样本和二抗/同型对照 |
FC
FC 推荐条件
| 推荐稀释 | 请参考验证图说明或咨询技术支持 |
|---|---|
| 建议样本/阳性对照 | 建议选择靶点高表达样本作为阳性对照 |
| 关键条件 | 如检测胞内靶点,需优化固定/通透条件,并设置同型对照 |
| 预期结果 | 预期阳性群体荧光信号相对阴性/同型对照右移 |
| 对照设置 | 建议设置阳性样本、阴性样本和二抗/同型对照 |
相关产品
查找相关产品 >>抗原信息
| 抗原信息 | 请咨询 技术支持 |
|---|---|
| 序列 | Email For Sequence |
靶点信息
| 研究背景 | Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFC and VEGFD. Plays an essential role in the regulation of angiogenesis, vascular development, vascular permeability, and embryonic hematopoiesis. Promotes proliferation, survival, migration and differentiation of endothelial cells. Promotes reorganization of the actin cytoskeleton. Isoforms lacking a transmembrane domain, such as isoform 2, may function as decoy receptors for VEGFA, VEGFC and/or VEGFD. Isoform 2 plays an important role as a negative regulator of VEGFA- and VEGFC-mediated lymphangiogenesis by limiting the amount of free VEGFA and/or VEGFC and by preventing their binding to FLT4. Modulates FLT1 and FLT4 signaling by forming heterodimers. Binding of vascular growth factors to isoform 1 leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate and the activation of protein kinase C. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, reorganization of the actin cytoskeleton and activation of PTK2/FAK1. Required for VEGFA-mediated induction of NOS2 and NOS3, leading to the production of the signaling molecule nitric oxide (NO) by endothelial cells. Phosphorylates PLCG1. Promotes phosphorylation of FYN, NCK1, NOS3, PIK3R1, PTK2/FAK1 and SRC |
|---|---|
| 基因 ID | 3134 |
| 基因名 | Kdr |
| Swiss | P35918 |
| 别名 | VEGF Receptor 2 (YD20041),VEGF Receptor 2 (YD20041) Rabbit mAb,Kdr,Fetal liver kinase 1,Kinase NYK,Protein-tyrosine kinase receptor flk-1,Flk-1,Flk1 |
资料与技术支持
验证数据
欢迎联系技术支持获取验证图与应用案例。
常见问题
当前页面标注应用包括 ICC/IF, FC,建议结合页面验证图和推荐稀释比例进行预实验优化。
可通过页面询价/留言入口提交货号和批号,技术支持会协助提供对应批次资料。
页面推荐条件可作为起始浓度,不同样本、固定方式和检测体系可能需要梯度优化。