Tetrazolium Red(2,3,5-Triphenyltetrazolium chloride; TPTZ) is used to visualize dehydrogenase enzyme activity; initially the tetrazolium solution is colorless but changes to red when it comes into contact with hydrogen.
Tetrazolium red is used in a biochemical viability test for seeds. The test relies on dehydrogenase enzymes to release hydrogen ions which subsequently reduce the colorless tetrazolium salt solution to a red compound called formazan. Living cells turn red while dead cells remain colorless.
CAS
298-96-4
中文名称
四氮唑红
分子量
334.80
形式
Solid
运输条件
Room temperature in continental US; may vary elsewhere.
保存条件
4°C, sealed storage, away from moisture and light
溶解性
In Vitro:
H2O : 50 mg/mL (149.34 mM; Need ultrasonic)
DMSO : 16.67 mg/mL (49.79 mM; Need ultrasonic)
配制储备液
浓度溶剂体积质量
1 mg
5 mg
10 mg
1 mM
2.9869 mL
14.9343 mL
29.8686 mL
5 mM
0.5974 mL
2.9869 mL
5.9737 mL
10 mM
0.2987 mL
1.4934 mL
2.9869 mL
*
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。
In Vivo:
请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂: