TRPV4 (YD35486) Rabbit mAb (货号:AYD16563)
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货号
AYD16563
靶点/基因
Trpv4
宿主
Rabbit
克隆性
Monoclonal
反应种属
Mouse, Rat
应用
IHC-P
暂无验证图
欢迎提交实验图片，或联系 技术支持 获取更多信息。
产品信息
应用指南
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抗原信息
靶点信息
资料与支持
常见问题
| 反应 | Mouse, Rat |
|---|---|
| 宿主 | Rabbit |
| 克隆性 | Monoclonal |
| 同种型 | IgG |
| 应用 | IHC-P |
| 推荐浓度 | |
| 理论分子量 | 98kDa/98kDa |
| 实测分子量 | |
| 形式 | Liquid |
| 保存条件 | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.75% BSA,50% glycerol,pH7.3. |
| 偶联物 | Unconjugated |
| 阳性对照 | |
| 细胞定位 | Cell membrane, Apical cell membrane, Cell junction, adherens junction, Cell projection, cilium |
| 纯化 | 亲和纯化 |
应用与推荐条件
快速判断怎么用
以下条件基于推荐浓度、验证图说明与通用实验要求整理，可作为预实验起点；不同样本和检测体系建议做梯度优化。
IHC-P
IHC-P 推荐条件
| 推荐稀释 | 请参考验证图说明或咨询技术支持 |
|---|---|
| 建议样本/阳性对照 | 建议选择靶点高表达样本作为阳性对照 |
| 关键条件 | 石蜡切片建议优化抗原修复液 pH、修复时间和一抗孵育条件 |
| 预期结果 | 预期定位：Cell membrane, Apical cell membrane, Cell junction, adherens junction, Cell projection, cilium |
| 对照设置 | 建议设置阳性样本、阴性样本和二抗/同型对照 |
相关产品
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抗原信息
| 抗原信息 | 请咨询 技术支持 |
|---|---|
| 序列 | Email For Sequence |
靶点信息
| 研究背景 | Non-selective calcium permeant cation channel involved in osmotic sensitivity and mechanosensitivity (PubMed:11094154, PubMed:12093812, PubMed:12538589). Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification (PubMed:12093812, PubMed:14691263, PubMed:16368742, PubMed:16571723). Also activated by heat, low pH, citrate and phorbol esters (PubMed:14691263). Increase of intracellular Ca(2+) potentiates currents. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism (By similarity). Acts as a regulator of intracellular Ca(2+) in synoviocytes (By similarity). Plays an obligatory role as a molecular component in the nonselective cation channel activation induced by 4-alpha-phorbol 12,13-didecanoate and hypotonic stimulation in synoviocytes and also regulates production of IL-8 (By similarity). Together with PKD2, forms mechano- and thermosensitive channels in cilium (PubMed:18695040). Promotes cell-cell junction formation in skin keratinocytes and plays an important role in the formation and/or maintenance of functional intercellular barriers (PubMed:20413591). Negatively regulates expression of PPARGC1A, UCP1, oxidative metabolism and respiration in adipocytes (PubMed:23021218). Regulates expression of chemokines and cytokines related to pro-inflammatory pathway in adipocytes (PubMed:23021218). Together with AQP5, controls regulatory volume decrease in salivary epithelial cells (PubMed:16571723). Required for normal development and maintenance of bone and cartilage (By similarity). In its inactive state, may sequester DDX3X at the plasma membrane. When activated, the interaction between both proteins is affected and DDX3X relocalizes to the nucleus (By similarity). In neurons of the central nervous system, could play a role in triggering voluntary water intake in response to increased sodium concentration in body fluid (PubMed:27252474) Non-selective calcium permeant cation channel involved in osmotic sensitivity and mechanosensitivity (PubMed:11081638). Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification (PubMed:11081638). Also activated by heat, low pH, citrate and phorbol esters (By similarity). Increase of intracellular Ca(2+) potentiates currents (By similarity). Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism (By similarity). Acts as a regulator of intracellular Ca(2+) in synoviocytes (By similarity). Plays an obligatory role as a molecular component in the nonselective cation channel activation induced by 4-alpha-phorbol 12,13-didecanoate and hypotonic stimulation in synoviocytes and also regulates production of IL-8 (By similarity). Together with PKD2, forms mechano- and thermosensitive channels in cilium (By similarity). Promotes cell-cell junction formation in skin keratinocytes and plays an important role in the formation and/or maintenance of functional intercellular barriers (By similarity). Negatively regulates expression of PPARGC1A, UCP1, oxidative metabolism and respiration in adipocytes (By similarity). Regulates expression of chemokines and cytokines related to pro-inflammatory pathway in adipocytes (By similarity). Together with AQP5, controls regulatory volume decrease in salivary epithelial cells (By similarity). Required for normal development and maintenance of bone and cartilage (By similarity). In its inactive state, may sequester DDX3X at the plasma membrane. When activated, the interaction between both proteins is affected and DDX3X relocalizes to the nucleus (By similarity). In neurons of the central nervous system, could play a role in triggering voluntary water intake in response to increased sodium concentration in body fluid (By similarity) |
|---|---|
| 基因 ID | 3134 |
| 基因名 | Trpv4 |
| Swiss | Q9EPK8, Q9ERZ8 |
| 别名 | TRPV4 (YD35486),TRPV4 (YD35486) Rabbit mAb,Trpv4,Osm-9-like TRP channel 4,Transient receptor potential protein 12,Vanilloid receptor-like channel 2,Vanilloid receptor-like protein 2,Vanilloid receptor-related osmotically-activated channel,Trp12,Vrl2,Vroac |
资料与技术支持
验证数据
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常见问题
当前页面标注应用包括 IHC-P，建议结合页面验证图和推荐稀释比例进行预实验优化。
可通过页面询价/留言入口提交货号和批号，技术支持会协助提供对应批次资料。
页面推荐条件可作为起始浓度，不同样本、固定方式和检测体系可能需要梯度优化。