生物活性 | Recombinant Proteinase K is a serine protease that cleaves the carboxy-terminated peptide bonds of aliphatic and aromatic amino acids. Recombinant Proteinase K can be used to digest proteins and remove contamination from nucleic acid preparations. |
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CAS | |
中文名称 | 重组蛋白酶 K |
分子量 | |
体外研究 | Proteinase K digestion1. Cells are resuspended in lysis bufferand finally lysed on ice for 15 minutes.2. The crude lysates are obtained by centrifugation at 4°C for 15 minutes. Proteinase K is added to a 20 µl aliquot to get the final concentrations of 2, 4 and 8 µg/ml. Reactions are carried out at 37°C for 30 minutes. The accuracy of these methods have not been independently confirmed. They are for reference only. |
体内研究 | |
形式 | Solid |
运输条件 | Room temperature in continental US; may vary elsewhere. |
保存条件 | -20°C, sealed storage, away from moisture |
溶解性 |
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说明书 |
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