| 生物活性 | L-ascorbic acid 2-phosphate (2-Phospho-L-ascorbic acid) magnesium hydrate is a long-acting vitamin C derivative that can stimulate collagen formation and expression. L-ascorbic acid 2-phosphate magnesium hydrate can be used as a culture medium supplement for the osteogenic differentiation of human adipose stem cells (hASCs). L-ascorbic acid 2-phosphate magnesium hydrate increases alkaline phosphatase (ALP) activity and expression of runx2A in hASCs during the osteogenic differentiation. | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| CAS | 1713265-25-8 | ||||||||
| 中文名称 | |||||||||
| 分子量 | |||||||||
| 体外研究 | L-Ascorbic acid 2-phosphate (0.1-1.5 mM; 2 to 3 weeks with medium exchange every 2 to 3 days) magnesium hydrate significantly stimulates cell growth, whereas addition of l-Ascorbic acid (Asc) achieves only weak growth stimulation. A combination of Asc-2P and bFGF significantly increases cell growth, but supplementation with EGF and/or insulin does not have any additional effect . L-Ascorbic acid 2-phosphate (50 µM-250 µM) magnesium hydrate is needed for the effective osteogenic differentiation of human adipose stem cells (hASCs), and higher concentrations of AsA2-P results in increased runx2 expression and ALP activity. The highest proliferation, ALP activity and runx2 expression is achieved with 150 µM AsA2-P and 10 nM dexamethasone (Dex), and 250 µM AsA2-P and 5 nM Dex. The accuracy of these methods have not been independently confirmed. They are for reference only. Cell Proliferation Assay
|
||||||||
| 体内研究 | |||||||||
| 形式 | |||||||||
| 运输条件 | Room temperature in continental US; may vary elsewhere. | ||||||||
| 保存条件 | Please store the product under the recommended conditions in the Certificate of Analysis. | ||||||||
| 溶解性 |
| 纯度 | |
|---|---|
| 说明书 |
暂无数据
