| 反应 | Human,Mouse,Rat |
|---|---|
| 宿主 | Mouse |
| 克隆性 | Monoclonal |
| 预测反应 | WB: Arabidopsis thaliana , Mus musculus , Rhinopithecus , Homo sapiens , Zika virus , Sus scrofa , Other , Rattus norvegicus , Carassius auratus , Gallus gallus , fenugreek , Hamster , Nicotiana tabacum L. , Chlorocebus aethiops , Gynura procumbens , Mesocricetus auratus , Canis lupus familiaris , Homo s , Chlorocebus sabaeus , Boswellia carterii , Capra hircus , Danio rerio , Ctenopharyngodon idellus , Giardia duodenalis , Ovis aries , T. granosa , Yeast , Fenneropenaeus chinensis IHC: Mus musculus , Homo sapiens , Rattus norvegicus IF: Homo sapiens , Chlorocebus sabaeus , Sus scrofa , Mus musculus IP: Homo sapiens , Mus musculus Co-IP: Homo sapiens WB，IF: Mus musculus WB，IHC，IF: Homo sapiens ELISA: Ctenopharyngodon idellus |
| 应用 | WBIHCIF/ICC |
| 推荐浓度 | WB: 1:2000 - 1:10000 IHC: 1:50 - 1:200 IF/ICC: 1:50 - 1:200 |
| 理论分子量 | 31kDa/36kDa |
| 实测分子量 | 36KDa |
| 形式 | Liquid |
| 保存条件 | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3. |
| 偶联物 | Unconjugated |
| 阳性对照 | HeLa,293T,HepG2,Mouse liver,Mouse spleen,Rat liver,Rat kidney |
| 细胞定位 | Cytoplasm,Membrane,Nucleus,cytoskeleton,cytosol,perinuclear region |
| 纯化 | Affinity purification |
| 抗原信息 | Recombinant fusion protein. |
|---|---|
| 序列 | Email For Sequence |
| 研究背景 | This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants. |
|---|---|
| 基因ID | 2597 |
| 基因名 | GAPDH |
| Swiss | P04406 |
| 别名 | G3PD;GAPD;HEL-S-162eP;GAPDH |
| 功能 | Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation. |
| 研究领域 |
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