Deoxycytidine triphosphate trisodium salt  (货号:B26734)

说明书
Deoxycytidine triphosphate trisodium salt
规格价格
100 mg 询价
200 mg 询价
10 mg ¥650.00 加购物车
50 mg ¥1550.00 加购物车

货号:B26734

生物活性 Deoxycytidine triphosphate trisodium salt (dCTP trisodium salt) is a nucleoside triphosphate that can be used for DNA synthesis. Deoxycytidine triphosphate trisodium salt has many applications, such as real-time PCR, cDNA synthesis, and DNA sequencing.
CAS 109909-44-6
中文名称 脱氧胞苷三磷酸三钠盐; 2′-脱氧胞苷-5′-三磷酸三钠盐
分子量 533.10
体外研究

Assays of thirteen cell lines, derived from mouse lymphomas, myelomas, myeloid tumors, and a mastocytoma, for sensitivity to growth inhibition by 1-β-D-arabinofuranosylcytosine (ara-C) revealed a spectrum between the most and least sensitive which differed 100-fold from each other. An inverse correlation between sensitivity and cellular Deoxycytidine triphosphate (2′-Deoxycytidine-5′-triphosphate, dCTP) content is found, and this suggested that sensitivity of cells might be increased if the dCTP content was lowered during cell exposure to ara-C.
Deoxycytidine triphosphate trisodium salt is a phosphoethanolamine cytidylyltransferase substrate.

The accuracy of these methods have not been independently confirmed. They are for reference only.

体内研究
形式 Solid
运输条件 Room temperature in continental US; may vary elsewhere.
保存条件 4°C, sealed storage, away from moisture
溶解性
In Vitro: 

H2O : 250 mg/mL (468.96 mM; Need ultrasonic)

DMSO : (insoluble or slightly soluble)

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM1.8758 mL9.3791 mL18.7582 mL
5 mM0.3752 mL1.8758 mL3.7516 mL
10 mM0.1876 mL0.9379 mL1.8758 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: PBS

    Solubility: 100 mg/mL (187.58 mM); Clear solution; Need ultrasonic

*以上所有助溶剂都可在 MCE 网站选购。

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