BRCA1 (YD14051) Rabbit mAb (货号:AYD15445)
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货号
AYD15445
靶点/基因
BRCA1
宿主
Rabbit
克隆性
Monoclonal
反应种属
Human
应用
IHC-P, ICC/IF
暂无验证图
欢迎提交实验图片，或联系 技术支持 获取更多信息。
产品信息
应用指南
相关产品
抗原信息
靶点信息
资料与支持
常见问题
| 反应 | Human |
|---|---|
| 宿主 | Rabbit |
| 克隆性 | Monoclonal |
| 同种型 | IgG |
| 应用 | IHC-PICC/IF |
| 推荐浓度 | |
| 理论分子量 | 208kDa |
| 实测分子量 | |
| 形式 | Liquid |
| 保存条件 | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.75% BSA,50% glycerol,pH7.3. |
| 偶联物 | Unconjugated |
| 阳性对照 | MCF-7 cells |
| 细胞定位 | Nucleus, Chromosome, Cytoplasm |
| 纯化 | 亲和纯化 |
应用与推荐条件
快速判断怎么用
以下条件基于推荐浓度、验证图说明与通用实验要求整理，可作为预实验起点；不同样本和检测体系建议做梯度优化。
IHC-P
IHC-P 推荐条件
| 推荐稀释 | 请参考验证图说明或咨询技术支持 |
|---|---|
| 建议样本/阳性对照 | MCF-7 cells |
| 关键条件 | 石蜡切片建议优化抗原修复液 pH、修复时间和一抗孵育条件 |
| 预期结果 | 预期定位：Nucleus, Chromosome, Cytoplasm |
| 对照设置 | 建议设置阳性样本、阴性样本和二抗/同型对照 |
ICC/IF
ICC/IF 推荐条件
| 推荐稀释 | 请参考验证图说明或咨询技术支持 |
|---|---|
| 建议样本/阳性对照 | MCF-7 cells |
| 关键条件 | 建议优化固定、通透和封闭条件，并设置二抗空白对照 |
| 预期结果 | 预期荧光定位：Nucleus, Chromosome, Cytoplasm |
| 对照设置 | 建议设置阳性样本、阴性样本和二抗/同型对照 |
相关产品
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抗原信息
| 抗原信息 | 请咨询 技术支持 |
|---|---|
| 序列 | Email For Sequence |
靶点信息
| 研究背景 | This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants, some of which are disease-associated mutations, have been described for this gene, but the full-length natures of only some of these variants has been described. A related pseudogene, which is also located on chromosome 17, has been identified. |
|---|---|
| 基因 ID | 672 |
| 基因名 | BRCA1 |
| Swiss | P38398 |
| 别名 | BRCA1 (YD14051),BRCA1 (YD14051) Rabbit mAb,BRCA1,RING finger protein 53,RING-type E3 ubiquitin transferase BRCA1,RNF53 |
资料与技术支持
验证数据
欢迎联系技术支持获取验证图与应用案例。
常见问题
当前页面标注应用包括 IHC-P, ICC/IF，建议结合页面验证图和推荐稀释比例进行预实验优化。
可通过页面询价/留言入口提交货号和批号，技术支持会协助提供对应批次资料。
页面推荐条件可作为起始浓度，不同样本、固定方式和检测体系可能需要梯度优化。