生物活性 | 4-Nitrophenyl phosphate (p-nitrophenyl phosphate) disodium hexahydrate is widely used as a small molecule phosphotyrosine-like substrate in activity assays for protein tyrosine phosphatases. 4-Nitrophenyl phosphate disodium hexahydrate is a colorless substrate that upon hydrolysis is converted to a yellow 4-nitrophenolate ion that can be monitored by absorbance at 405 nm. | ||||||||||||||||
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CAS | 333338-18-4 | ||||||||||||||||
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分子量 | 371.14 | ||||||||||||||||
体外研究 | 4-Nitrophenyl phosphate (PNPP) disodium hexahydrate is a commonly used substrate for alkaline phosphatases (ALPs). 4-Nitrophenyl phosphate disodium hexahydrate is hydrolyzed by ALP to PNP (p-nitrophenol), which quenches the fluorescence of novel gold nanoclusters (AuNCs) by the inner filter effect (IFE). The accuracy of these methods have not been independently confirmed. They are for reference only. |
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形式 | Solid | ||||||||||||||||
运输条件 | Room temperature in continental US; may vary elsewhere. | ||||||||||||||||
保存条件 | -20°C, sealed storage, away from moisture | ||||||||||||||||
溶解性 | In Vitro: H2O : 100 mg/mL (269.44 mM; Need ultrasonic) DMSO : 10 mg/mL (26.94 mM; ultrasonic and warming and heat to 80°C) 配制储备液
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