4-Nitrophenyl phosphate disodium hexahydrate  (货号:B26831)

说明书
4-Nitrophenyl phosphate disodium hexahydrate
规格价格
Free Sample (0.1 - 0.2 mg) 询价
5 g 询价
10 g 询价
1 g ¥300.00 加购物车

货号:B26831

生物活性 4-Nitrophenyl phosphate (p-nitrophenyl phosphate) disodium hexahydrate is widely used as a small molecule phosphotyrosine-like substrate in activity assays for protein tyrosine phosphatases. 4-Nitrophenyl phosphate disodium hexahydrate is a colorless substrate that upon hydrolysis is converted to a yellow 4-nitrophenolate ion that can be monitored by absorbance at 405 nm.
CAS 333338-18-4
中文名称
分子量 371.14
体外研究

4-Nitrophenyl phosphate (PNPP) disodium hexahydrate is a commonly used substrate for alkaline phosphatases (ALPs). 4-Nitrophenyl phosphate disodium hexahydrate is hydrolyzed by ALP to PNP (p-nitrophenol), which quenches the fluorescence of novel gold nanoclusters (AuNCs) by the inner filter effect (IFE).

The accuracy of these methods have not been independently confirmed. They are for reference only.

体内研究
形式 Solid
运输条件 Room temperature in continental US; may vary elsewhere.
保存条件 -20°C, sealed storage, away from moisture
溶解性
In Vitro: 

H2O : 100 mg/mL (269.44 mM; Need ultrasonic)

DMSO : 10 mg/mL (26.94 mM; ultrasonic and warming and heat to 80°C)

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM2.6944 mL13.4720 mL26.9440 mL
5 mM0.5389 mL2.6944 mL5.3888 mL
10 mM0.2694 mL1.3472 mL2.6944 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

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